3.1 The genomic sequence of Rhizobium rhizogenes strain LBA9402
As Illumina sequencing data alone were not sufficient to obtain a high quality and complete genome sequence of strain LBA9402, we additionally obtained long reads by Nanopore sequencing. Unicycler was used to obtain a hybrid assembly. This resulted in three circular contigs of 3,958,212 bp, 2,005,144 bp and 252,168 bp, respectively. More than 99.9% of both MinION and Illumina reads align to the assembly, indicating that the assembly is complete. The G+C content of the genome was 60%. The assembly was annotated using NCBI Prokaryotic Genome Annotation Pipeline (PGAP). In total, 5822 coding sequences, 9 rRNA genes (3 operons), and 53 tRNA genes were annotated. COG categories were assigned to predicted coding sequences with eggNOG-mapper (supplementary figure S1, Supplementary Material online).