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Recombinant PreS-fusion protein vaccine for birch pollen and apple allergy
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  • Musa Khaitov,
  • Igor Shilovskiy ,
  • Rudolf Valenta,
  • Milena Weber,
  • Korneev Artem,
  • Inna Tulaeva,
  • Pia Gattinger,
  • Marianne van Hage,
  • Gerhard Hofer,
  • Jon Konradsen,
  • Walter Keller,
  • Oluwatoyin Akinfenwa,
  • Alina Poroshina,
  • Nataliya Ilina,
  • Elena Fedenko,
  • Olga G. Elisyutina,
  • Alla Litovkina,
  • Evgenii V. Smolnikov,
  • Alexandra Nikonova,
  • Sergei Rybalkin,
  • Vladimir Aldobaev,
  • Valeriy Smirnov,
  • Nadezhda Shershakova,
  • Olga Petukhova,
  • Dmitry Kudlay,
  • Artem Shatilov ,
  • Anastasia Timofeeva ,
  • Raffaela Campana,
  • Sevastyan Rabdano,
  • Victor Trukhin,
  • Sergey Udin,
  • Veronica Skvortsova
Musa Khaitov
FGBU Gosudarstvennyj naucnyj centr Institut immunologii Federal'nogo mediko-biologiceskogo agentstva Rossii

Corresponding Author:[email protected]

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Igor Shilovskiy
FGBU Gosudarstvennyj naucnyj centr Institut immunologii Federal'nogo mediko-biologiceskogo agentstva Rossii
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Rudolf Valenta
FGBU Gosudarstvennyj naucnyj centr Institut immunologii Federal'nogo mediko-biologiceskogo agentstva Rossii
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Milena Weber
Medizinische Universitat Wien Zentrum fur Pathophysiologie Infektiologie und Immunologie
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Korneev Artem
FGBU Gosudarstvennyj naucnyj centr Institut immunologii Federal'nogo mediko-biologiceskogo agentstva Rossii
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Inna Tulaeva
Medizinische Universitat Wien Zentrum fur Pathophysiologie Infektiologie und Immunologie
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Pia Gattinger
Medizinische Universitat Wien Zentrum fur Pathophysiologie Infektiologie und Immunologie
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Marianne van Hage
Karolinska Institutet Institutionen for medicin Solna
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Gerhard Hofer
Stockholms universitet Lararhogskolan i Stockholm
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Jon Konradsen
Karolinska Institutet Institutet for miljomedicin
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Walter Keller
BioTechMed-Graz Geschaftsstelle
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Oluwatoyin Akinfenwa
Medizinische Universitat Wien Zentrum fur Pathophysiologie Infektiologie und Immunologie
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Alina Poroshina
FGBU Gosudarstvennyj naucnyj centr Institut immunologii Federal'nogo mediko-biologiceskogo agentstva Rossii
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Nataliya Ilina
FGBU Gosudarstvennyj naucnyj centr Institut immunologii Federal'nogo mediko-biologiceskogo agentstva Rossii
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Elena Fedenko
FGBU Gosudarstvennyj naucnyj centr Institut immunologii Federal'nogo mediko-biologiceskogo agentstva Rossii
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Olga G. Elisyutina
FGBU Gosudarstvennyj naucnyj centr Institut immunologii Federal'nogo mediko-biologiceskogo agentstva Rossii
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Alla Litovkina
FGBU Gosudarstvennyj naucnyj centr Institut immunologii Federal'nogo mediko-biologiceskogo agentstva Rossii
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Evgenii V. Smolnikov
FGBU Gosudarstvennyj naucnyj centr Institut immunologii Federal'nogo mediko-biologiceskogo agentstva Rossii
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Alexandra Nikonova
FGBU Gosudarstvennyj naucnyj centr Institut immunologii Federal'nogo mediko-biologiceskogo agentstva Rossii
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Sergei Rybalkin
Rossijskij nacional'nyj issledovatel'skij medicinskij universitet imeni N I Pirogova
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Vladimir Aldobaev
Rossijskij nacional'nyj issledovatel'skij medicinskij universitet imeni N I Pirogova
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Valeriy Smirnov
FGBU Gosudarstvennyj naucnyj centr Institut immunologii Federal'nogo mediko-biologiceskogo agentstva Rossii
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Nadezhda Shershakova
FGBU Gosudarstvennyj naucnyj centr Institut immunologii Federal'nogo mediko-biologiceskogo agentstva Rossii
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Olga Petukhova
FGBU Gosudarstvennyj naucnyj centr Institut immunologii Federal'nogo mediko-biologiceskogo agentstva Rossii
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Dmitry Kudlay
FGBU Gosudarstvennyj naucnyj centr Institut immunologii Federal'nogo mediko-biologiceskogo agentstva Rossii
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Artem Shatilov
FGBU Gosudarstvennyj naucnyj centr Institut immunologii Federal'nogo mediko-biologiceskogo agentstva Rossii
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Anastasia Timofeeva
FGBU Gosudarstvennyj naucnyj centr Institut immunologii Federal'nogo mediko-biologiceskogo agentstva Rossii
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Raffaela Campana
Medizinische Universitat Wien Zentrum fur Pathophysiologie Infektiologie und Immunologie
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Sevastyan Rabdano
St Petersburg Research Institute of Vaccines and Serums of the Federal Medical Biological Agency
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Victor Trukhin
St Petersburg Research Institute of Vaccines and Serums of the Federal Medical Biological Agency
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Sergey Udin
Federal State Budgetary Institution “Centre for Strategic Planning and Management of Biomedical Health Risks” of the Federal Medical Biological Agency
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Veronica Skvortsova
Federal'noe mediko-biologiceskoe agentstvo Rossii
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Abstract

Background: Respiratory birch pollen allergy and associated oral allergy syndrome affect more than 150 million people. IgE cross-sensitization to major birch pollen allergen Bet v 1 and pathogenesis-related (PR10) plant food allergens is responsible for the pollen-food allergy syndrome. Methods: We designed a recombinant protein, AB-PreS, consisting of non-allergenic peptides derived from the IgE binding sites of Bet v 1 and the cross-reactive apple allergen, Mal d 1, fused to the PreS domain of HBV surface protein as immunological carrier. AB-PreS was expressed in E. coli and purified by chromatography. The allergenic activity of AB-PreS was tested using sera and basophils from birch pollen patients allergic. The protective effect of AB-PreS was assessed by inhibition ELISA test using sera allergic patients and from immunized rabbits. Results: IgE-binding experiments and basophil activation test revealed the hypoallergenic nature of AB-PreS. IgG antibodies induced by 5 injections with AB-PreS inhibited allergic patients’ IgE binding to Bet v 1 and Mal d 1 better than did IgG induced by up to 30 injections of six licensed birch pollen allergen extract-based vaccines. Additionally, immunization with AB-PreS induced HBV-specific antibodies potentially protecting the infection. Conclusion: The recombinant AB-PreS-based vaccine is hypoallergenic, safe and superior to currently registered allergen extract-based vaccines for the treatment of the birch pollen food allergy syndrome.
21 Apr 2023Submitted to Allergy
21 Apr 2023Submission Checks Completed
21 Apr 2023Assigned to Editor
21 Apr 2023Review(s) Completed, Editorial Evaluation Pending
24 Apr 2023Reviewer(s) Assigned
26 May 2023Editorial Decision: Revise Minor
13 Sep 20231st Revision Received
13 Sep 2023Submission Checks Completed
13 Sep 2023Assigned to Editor
13 Sep 2023Review(s) Completed, Editorial Evaluation Pending
15 Sep 2023Reviewer(s) Assigned
25 Sep 2023Editorial Decision: Accept