Proximity of the Macquarie perch and golden perch sex-linked SNPs
to known sex-determining genes
Forty-four Macquarie perch genes (Appendix D, Table D1) and 45 golden
perch genes (Table D2) found by BRAKER2 were annotated to genes or gene
families that act as sex-determining genes in other fish species. Of
these, one Macquarie perch gene (g10286 on scaffold 633, bases
131251-132300) and three golden perch genes (g1522, g23888 and g30513;
Table D5), all annotated to the SOX gene family, were on the
scaffolds containing sex-linked DArT SNPs and the 146-bp Macquarie perch
sexing region. Three of these SOX genes- Macquarie perch gene
g10286 and golden perch genes g1522 and g23888- were annotated to a
function of regulating DNA-templated transcription (GO:0006355) and had
best Blastn hits to SOX1b , SOX1a and SOX14 of other
bony fishes, respectively (Appendix E). This suggests a role of theSOX transcription factor gene family regulating the fate of stem
and progenitor cells during development (Kiefer, 2007), in sex
determination of both perch species, with SOX1 gene being the
strongest candidate.