2.1 Sample Collection and DNA Extraction
Adult specimens ofAmipittia
virgate Leech, 1890 andHalpe
nephele Leech, 1893 were sampled at Jiuxian Mountain and Wuyi Mountain
in Fujian Province, China, respectively, in July, 2016. The adultOnryza maga Leech, 1890 were collected at Matou Mountain, Jiangxi
Province, China in August, 2018. Specimen identification was based on
morphological characteristics following Yuan et al. (Chou, 1994; Yuan et
al., 2015) and the identity was confirmed via cox1 barcoding
using the BOLD database (Ratnasingham & Hebert, 2013). All genetic
materials were immediately preserved in 100% ethanol immediately after
collecting and stored at -20˚C at the Entomological Museum of the
Northwest A&F University, Yangling, Shaanxi Province, China. The
genomic DNA was isolated from the thoracic tissue using the
EasyPureR Genomic DNA Kit (TransGen Biotech, Beijing).