2.1 Sample Collection and DNA Extraction
Adult specimens ofAmipittia virgate Leech, 1890 andHalpe nephele Leech, 1893 were sampled at Jiuxian Mountain and Wuyi Mountain in Fujian Province, China, respectively, in July, 2016. The adultOnryza maga Leech, 1890 were collected at Matou Mountain, Jiangxi Province, China in August, 2018. Specimen identification was based on morphological characteristics following Yuan et al. (Chou, 1994; Yuan et al., 2015) and the identity was confirmed via cox1 barcoding using the BOLD database (Ratnasingham & Hebert, 2013). All genetic materials were immediately preserved in 100% ethanol immediately after collecting and stored at -20˚C at the Entomological Museum of the Northwest A&F University, Yangling, Shaanxi Province, China. The genomic DNA was isolated from the thoracic tissue using the EasyPureR Genomic DNA Kit (TransGen Biotech, Beijing).