Figure 3 - Temperature rise over time determined experimentally (blue line) and estimated with the finite element simulation (orange line). a) 1.5 mL microcentrifuge tube with 1.5 mL sample at 50% amplitude using a 3mm tip (corresponding to ~5.5 Watt) and 10 second on and 10 second off pulse, initial temperature 1.6C. b) 1.5 mL microcentrifuge tube with 1.5 mL sample at 50% amplitude using a 3mm tip (corresponding to ~5.5 Watt) and 20 second on and 20 second off pulse, initial temperature 3.4C. c) 1.5 mL microcentrifuge tube with 1.5 mL sample at 25% amplitude using a 3mm tip (corresponding to ~2 Watt) and 10 second on and 10 second off pulse, initial temperature 1.1C. d) 5 mL microcentrifuge tube with 5 mL sample at 50% amplitude using a 6mm tip (corresponding to ~12.5 Watt) and 20 second on and 20 second off pulse, initial temperature at 14.4 C.