6 Figure legend
Figure 1 - Disease
severity distribution of B. cinerea on leaves from root-treated A.
thaliana plants grown hydroponically. Treatments included control and
two oxylipins: 13-HPOD and 13-HPOT. Scoring: 1, no symptoms; scoring 2,
lesions smaller than 0.5 cm; scoring 3, lesions larger than 0.5 cm;
scoring 4, beginning of sporulation on lesions. Results are based on
three independent experiments (n=3). Standard deviation between the
experiments is less than 7%.
Figure 2 - Early defense responses detection induced by HPOs inA. thaliana cells suspensions. (A, B) Production of ROS
by controls and HPOs treated cells. (A) Mean area under
H2O2 production curves for 90 min
measurements. Data are based on three independent repetitions (n=3) and
error bars are the standard deviations of means. (B) One
example of kinetics of ROS production for 13-HPOT, 13-HPOD, MS negative
control and COS-OGA positive control.
Figure 3 - Evolution of Laurdan generalized polarization as a
function of temperature for PLPC:sito:GluCer MLVs (50 µM) in the absence
or presence of HPOs (lipid:HPO molar ratio 5:1).
Figure 4 - (A,B) Reflectivity curves (symbols),
relatives fits (lines) and (C,D) obtained SLD profiles of the
d62DPPC membrane investigated in two contrasts before
(green and orange) and after (bleu and red) the addition of 13-HPOT
(left) and 13-HPOD (right) (lipid:HPO molar ratio 5:1). (E) Fit
parameters of the d62DPPC SLB alone and after the
interaction with 13-HPOD/T. Parameters (Thickness, scattering length
density (SLD), solvent penetration (Solv p) and roughness) correspond to
a contemporary fit performed on H2O and
D2O solutions with 10mM Tris buffer (pH 7.4). Errors
have been estimated by changing the parameters up to a variation of two
in the χ2. For each parameter, the maximum error found
was kept. Measurements were carried out at room temperature.
Figure 5 - HPOs lead to lipids erosion. A) AFM
topographic image of a PLPC:sito:GluCer (60:20:20) supported lipid
bilayer (SLB), deposited on mica, and recorded in 10 mM Tris buffer at
pH 7.5. B) Height profile of the SLB along the dashed line in
(A) allowing the measurement of the sample thickness e. C)Force curves recorded on a bright area in (A) confirming the presence of
the SLB, with a thickness e. D) AFM topographic images before
(t0) and after injection of 13-HPOT or 13-HPOD, at
increasing incubation times. Scale bar 1 µm, same colour scale than in
(A). E-F) Height density profiles recorded on small areas,
defined as dashed squares in (D), of the PLPC:sito:GluCer bilayer before
and after 75 min - incubation with 13-HPOT and 13-HPOD respectively.