6 Figure legend
Figure 1 - Disease severity distribution of B. cinerea on leaves from root-treated A. thaliana plants grown hydroponically. Treatments included control and two oxylipins: 13-HPOD and 13-HPOT. Scoring: 1, no symptoms; scoring 2, lesions smaller than 0.5 cm; scoring 3, lesions larger than 0.5 cm; scoring 4, beginning of sporulation on lesions. Results are based on three independent experiments (n=3). Standard deviation between the experiments is less than 7%.
Figure 2 - Early defense responses detection induced by HPOs inA. thaliana cells suspensions. (A, B) Production of ROS by controls and HPOs treated cells. (A) Mean area under H2O2 production curves for 90 min measurements. Data are based on three independent repetitions (n=3) and error bars are the standard deviations of means. (B) One example of kinetics of ROS production for 13-HPOT, 13-HPOD, MS negative control and COS-OGA positive control.
Figure 3 - Evolution of Laurdan generalized polarization as a function of temperature for PLPC:sito:GluCer MLVs (50 µM) in the absence or presence of HPOs (lipid:HPO molar ratio 5:1).
Figure 4 - (A,B) Reflectivity curves (symbols), relatives fits (lines) and (C,D) obtained SLD profiles of the d62DPPC membrane investigated in two contrasts before (green and orange) and after (bleu and red) the addition of 13-HPOT (left) and 13-HPOD (right) (lipid:HPO molar ratio 5:1). (E) Fit parameters of the d62DPPC SLB alone and after the interaction with 13-HPOD/T. Parameters (Thickness, scattering length density (SLD), solvent penetration (Solv p) and roughness) correspond to a contemporary fit performed on H2O and D2O solutions with 10mM Tris buffer (pH 7.4). Errors have been estimated by changing the parameters up to a variation of two in the χ2. For each parameter, the maximum error found was kept. Measurements were carried out at room temperature.
Figure 5 - HPOs lead to lipids erosion. A) AFM topographic image of a PLPC:sito:GluCer (60:20:20) supported lipid bilayer (SLB), deposited on mica, and recorded in 10 mM Tris buffer at pH 7.5. B) Height profile of the SLB along the dashed line in (A) allowing the measurement of the sample thickness e. C)Force curves recorded on a bright area in (A) confirming the presence of the SLB, with a thickness e. D) AFM topographic images before (t0) and after injection of 13-HPOT or 13-HPOD, at increasing incubation times. Scale bar 1 µm, same colour scale than in (A). E-F) Height density profiles recorded on small areas, defined as dashed squares in (D), of the PLPC:sito:GluCer bilayer before and after 75 min - incubation with 13-HPOT and 13-HPOD respectively.