MATERIALS AND METHODS
The materials and methods used in seed train process (cell line and
media used); production process and bioreactor operations (14-day
fed-batch culture); transcriptome, proteome and metabolome profiling of
CHO cells; IgG quality attribute analyses (purification of harvest
filtrate; N-glycome, charge variant and aggregation analyses), and
computational analysis of multi-omics data, and the determination of
specific growth and metabolite consumption/production rates, are
presented in Supplementary Methods .