MATERIALS AND METHODS

The materials and methods used in seed train process (cell line and media used); production process and bioreactor operations (14-day fed-batch culture); transcriptome, proteome and metabolome profiling of CHO cells; IgG quality attribute analyses (purification of harvest filtrate; N-glycome, charge variant and aggregation analyses), and computational analysis of multi-omics data, and the determination of specific growth and metabolite consumption/production rates, are presented in Supplementary Methods .