3.1. Response of endophytic microbial inoculum on soil physico-chemical properties
From the previous experimental result and compatibility test, we have selected two seed endophytic bacteria for preparing the consortia (T3). These are Enterobacter hormaechei BHUJPCS-15 (Accession number MN078052) (T1) and Enterobacter cloacae BHUJPCS-21 (Accession number MN078055) (T2). We have found that microbial consortia have the ability to produce IAA (61.04 µg/ml), ammonia (105.45 µg/ml), siderophore (18.06) and solubilize essential nutrient like phosphate (871.14 µg/ml) and potassium, and showed antagonistic activity against chickpea pathogen Fusarium sp. in-vitro condition(Table. 1) . After showing positive result in PGP biochemical activity we set an experiment for chickpea growth promotion with the use of these two seed endophytes and its consortia. Experiment was done in pot and field condition to check microbial effect on soil health and yield (Fig. 1).
The physiochemical property of experimental soil was checked and recorded, the pH of pot and field soil were alkaline and the range varied from 7.09 to 8.08 and 8.01 to 8.52 respectively, there was no significant change in pH of pot and field trial. Other physiochemical property such as EC ranged from 15.04 to 19.12 (us/cm) and 25.00 to 28.14 (us/cm) in pot and field soil respectively, and the significant change was showed in all three treatment Control (T0)>E cloacae BHUJPCS-21 (T2)> E hormaecheiBHUJPCS-15 (T1)> Consortia (T3) in compare with untreated control both in pot and field in same manner (Table: 2).Organic matter (OM) and organic carbon (OC) also recorded and the rage was varied from 1.86 to 2.18 and 1.07 to1.32 respectively in pot soil and the significant increase in % OM and % OC was observed in Control > E cloacae BHUJPCS-21 > E hormaechei BHUJPCS-15 > Consortia order, and % OM and % OC was recorded 1.28 to 1.87 and 25.00 to 28.14 in experimental field in Control> E hormaechei BHUJPCS-15>E cloacae BHUJPCS-21> Consortia and Control> E cloacae BHUJPCS-21> E hormaechei BHUJPCS-15> Consortia respectively