3.4. Contamination Assessment Test
Since multi-well plate format was used, it is possible the additional washing steps may introduce cross-contamination. We assessed the contamination risk of MADCAT assay (Figure 3). The results showed that even if 24 high-concentration samples were processed in the same plate, they did not contaminate a negative well surrounded by them, nor did a high-concentration sample well bring contamination to any negative well surrounding it. Given the high sensitivity of the assay, such results indicate very low probability of cross-contamination.