A panel of 48 clinically validated field samples (22 of serum, 21 of whole blood, and 5 of tissue homogenates from heart, spleen, kidney, lymph, and lung) were tested to further verify the clinical performance of the MADCAT assay. The experiment was performed at African Swine Fever Regional Laboratory of China (Lanzhou), Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences under ethical approval. There was 100% agreement between the results of MADCAT method and OIE-recommended qPCR method (Table 2). In addition, lower Ct values were obtained with our method compared to OIE-recommended qPCR when detecting the ASFV-infected samples (Table 3).
Table 2. Comparison between the OIE-recommended qPCR and the MADCAT assay for the detection of ASFV among real clinical samples