<div>A panel of 48 clinically validated field samples (22 of serum, 21 of
whole blood, and 5 of tissue homogenates from heart, spleen, kidney,
lymph, and lung) were tested to further verify the clinical performance
of the MADCAT assay. The experiment was performed at African Swine Fever
Regional Laboratory of China (Lanzhou),
Lanzhou Veterinary Research
Institute, Chinese Academy of Agricultural Sciences under ethical
approval. There was 100% agreement between the results of MADCAT method
and OIE-recommended qPCR method (Table 2). In addition, lower Ct values
were obtained with our method compared to OIE-recommended qPCR when
detecting the ASFV-infected samples (Table 3).</div><div><b>Table 2.</b> Comparison between the OIE-recommended qPCR and the
MADCAT assay for the detection of ASFV among real clinical samples</div>