2.1. Standard DNA, Primers and Probes
Plasmid carrying a 415 bp highly conserved sequence of ASFV VP72 gene
based on the sequence alignment of 137 strains using MAFFT version 7 was
synthesized (BioTeke) covering regions of the designed capture probes
and the TaqMan amplicon. The plasmid was quantified with UV-Vis
spectrophotometers (Thermo Scientific™ NanoDrop 2000) and linearized by
QuickCut™ EcoR Ⅰ (Takara). All primers and probes sequences, as
listed in Table S1, were synthesized by Invitrogen.