2.2. Clinical Samples
Clinical samples, including serum, EDTA-blood, spleen, heart, kidney, lymph and lung were collected from domestic pigs in China. A panel of 22 positive and 26 negative samples were included in this study and provided by African Swine Fever Regional Laboratory of China (Lanzhou), Lanzhou Veterinary Research Institute, CAAS. The tissue samples were ground in a tissue homogenize and prepared a cell suspension at 10% with phosphate-buffered saline (1g tissue/10 ml sterile PBS).