2.1. Standard DNA, Primers and Probes
Plasmid carrying a 415 bp highly conserved sequence of ASFV VP72 gene based on the sequence alignment of 137 strains using MAFFT version 7 was synthesized (BioTeke) covering regions of the designed capture probes and the TaqMan amplicon. The plasmid was quantified with UV-Vis spectrophotometers (Thermo Scientific™ NanoDrop 2000) and linearized by QuickCut™ EcoR Ⅰ (Takara). All primers and probes sequences, as listed in Table S1, were synthesized by Invitrogen.