2.4 Citrullinated histone-3 detection
Pellets of BAL neutrophils (1 x 105) were lysed with RIPA buffer. Pools of BAL supernatants or of cell lysates from all animals per each group of treatment were analysed by Western blot using an anti-Histone H3 (citrulline R2+R8+R17) (ab5103Abcam, Cambridge, Mass., USA).