2.8 Gel filtration chromatography
The soluble proteins of the CRCT overexpression line in the crude
extract with buffer A (100 μL) were separated by gel filtration
chromatography using the SMART system (GE Healthcare) with a Superose 12
PC3.2/30 column equilibrated with buffer A containing 100 mM NaCl.
Separation was performed at a flow rate of 40 μL
min-1. Fractions (40 μL) were collected and subjected
to western blot analysis.