2.7 ChIP analysis
ChIP experiments were performed as described by Buzas et al. (2011). The non-crosslinked leaf sheath of the CRCT-FLAG overexpression line was used for this experiment. FLAG-CRCT associated DNA was immunoprecipitated using anti-DYKDDDDK tag antibody beads (Fujifilm Wako Chemical). Immunoprecipitated DNAs and input DNAs were purified using QIAquick PCR Purification Kit (Qiagen). ChIP-qPCR was performed using gene-specific primers (Table S1). Purified immunoprecipitated DNAs and input DNAs were used as templates, and qPCR was carried out using TBgreen Premix Ex Tag GC (Takara) and a thermal cycler (MyGoPro, IT-IS Life Science Ltd.) according to the manufacturer’s instructions. The 18S ribosomal RNA gene (XR_003238822) was examined as a reference.