2.8 Gel filtration chromatography
The soluble proteins of the CRCT overexpression line in the crude extract with buffer A (100 μL) were separated by gel filtration chromatography using the SMART system (GE Healthcare) with a Superose 12 PC3.2/30 column equilibrated with buffer A containing 100 mM NaCl. Separation was performed at a flow rate of 40 μL min-1. Fractions (40 μL) were collected and subjected to western blot analysis.