2.2 qRT-PCR Analyses
Total RNA was isolated from rice tissues using an RNeasy Plant Mini Kit (Qiagen, http://www.qiagen.com) according to the manufacturer’s instructions. The first strand cDNA was synthesized from total RNA with an oligo (dT)18 and a random hexamer as primers using PrimeScript II 1st Strand cDNA Synthesis Kit (Takara, http://www.takara-bio.co.jp). The qRT-PCR was performed using gene-specific primers (Table S1), TBgreen Premix Ex Tag GC (Takara) and a thermal cycler (MyGoPro, IT-IS Life Science Ltd., http://www.itislifescience.com). Expression of the Actin was examined as an internal control.