3.7. Transcriptional activation activity of CRCT
Transcriptional activation activity of CRCT was evaluated using fusions
of various length of CRCT with a Gal4 DNA-binding domain in yeast
(Figure S9). In this experiment, the growth of yeast in
SD2- plate (-Trp, -His) indicates the activation of
transcription of reporter gene. Transcriptional activation activity of
CRCT was detected at N-terminal regions, but not at C-terminal region of
CRCT, i.e., CCT domain. These results demonstrate that CRCT should be a
transcriptional activator.