Figure 5. Functional Comparison of Glomerular and Proximal
Convoluted Tubule (PCT) MPS in Single and Tri-culture Conditions (n=3
microsphyiological system [MPS] devices/condition). Figures A,C,E
represent single culture experimental tests with no glomerular cells,
and figures B, D, F depict a tri-culture MPS with a glomerulus housing
unit. (A) Filtrate output formation in single culture. (B) Filtrate
output formation in tri-culture. (C) Glucose secretion in single
culture. (D) Glucose secretion in tri-culture. (E) Protein filtration of
FITC- human serum albumin (HSA) in single culture. (** Comparison of
individual datapoints within urine output, p<0.01,
non-parametric one-way ANOVA). (F) Protein filtration of FITC-HSA in
tri-culture. (*** Comparison of individual datapoints within blood
circuit and filtrate output, p<0.001, non-parametric one-way
ANOVA and Mann-Whitney test). (G) HSA concentration of filtrate output
in single and tri-culture. (*** Comparison of rates in filtrate between
single and tri-cultures, p<0.001, linear regression,
Rsingle2= 0.86,
Rtri2= 0.20).