Figure 4. Simulated and Experimental Comparison of Glomerulus and Proximal Convoluted Tubule (PCT) MPS Pump Flow and Shear Stress. (A) Measured filtrate output flow (μL/min) for different total output pump flow (μL/min) in both simulated and experimental settings (0.25 mm ID tubing only). R2=1.0. (B) Shear stress across the apical PCT (dyne-s/cm2) for different total output pump flow (μL/min) in both simulated and experimental settings (0.25 mm ID tubing only). R2=1.0. (C) Scatter plot of simulated, experimental (single inlet tubing), single-culture, and tri-culture urine output flow under Qin= 45.3 μL/min. (D) Scatter plot of simulated, experimental (single inlet tubing), single-culture, and tri-culture shear stress (dyne-s/cm2) under Qin= 45.3 μL/min. (E) Assembled MPS flow validation. Line represents median. (* Comparison of datapoints within conditional test, p<0.05, non-parametric one-way ANOVA).