2.3 Cell preparation for transmission electron microscopy (TEM)
BHK-21 cells infected with SVV-CH-09-2018 for 18 h, were washed twice
with pre-cooled PBS and fixed with glutaraldehyde at 4°C. The cells were
recovered by scraping and were centrifuged at 12000 g for 10 min. The
supernatant was discarded. The cells were post-fixed in
OsO4, embedded in epoxy resin, and polymerized at 80°C
for at least 3 days. Finally, the cell samples were cut into 60-nm
slices and stained with uranyl acetate(Malenovska, 2013).The sections
mounted on grids were observed by TEM, using a model HT7600 microscope
(Hitachi, Japan).