Electron microscopy
Physical stability and integrity of the mosaic
CuMVTT-RBM were visualized by transmission electron
microscopy (Philips CM12 EM). For imaging, sample-grids were glow
discharged and 2µl of purified CuMVTT-RBM (3mg/ml) was
added for 30s. Grids were washed 3x with ddH2O and
negatively stained with 5 µl of 5% uranyl acetate for 30s. Excess
uranyle acetate was removed by pipetting and the grids were air dried
for 10min. Images were taken with 84,000x and 110,000x magnification.