Electron microscopy
Physical stability and integrity of the mosaic CuMVTT-RBM were visualized by transmission electron microscopy (Philips CM12 EM). For imaging, sample-grids were glow discharged and 2µl of purified CuMVTT-RBM (3mg/ml) was added for 30s. Grids were washed 3x with ddH2O and negatively stained with 5 µl of 5% uranyl acetate for 30s. Excess uranyle acetate was removed by pipetting and the grids were air dried for 10min. Images were taken with 84,000x and 110,000x magnification.