Modeling outline
The outline of our modeling workflow in CASP14 (Figure 1) was largely the same as in CASP1317, but with several improvements. During the initial step, for every target we attempted to identify multimeric templates for comparative (homology) modeling using sequence- and structure-based search methods. If the identified templates were not reliable, we used template-based docking. Coiled-coil protein structures were predicted using a custom designed procedure. If no templates could be identified, we switched to free docking. In the case of large multisubunit targets, we combined all the methods, employing comparative modeling approach for the parts of the complex for which templates were available followed by docking of the resulting subcomplexes to obtain the full assembly.