|
Structural element
|
Structural element
|
Structural element
|
Branching
(mU/mg)
|
Branching
(mU/mg)
|
Hydrolytic
(mU/mg)
|
B/H&
|
|
Loop |
Tyrosine |
β sheets |
|
|
|
|
Wild-type enzyme |
|
|
|
|
|
|
|
Thermus thermophilus HB8 |
+ |
+ |
+ |
480.0 ± 3.5#
|
480.0 ± 3.5#
|
11.7
± 1.5 |
41.0 ± 2.1 |
Thermococcus kodakaraensis KOD1 |
+ |
+ |
+ |
550.0 ± 5.5 |
550.0 ± 5.5 |
12.6 ± 1.7 |
43.6 ±
3.3 |
Calidithermus timidus DSM 17022 |
+ |
+ |
+ |
356.9 ± 10.6 |
356.9 ± 10.6 |
15.3 ± 2.5 |
24.1 ±
4.6 |
Thermoanaerobaculum aquaticum MP-01 |
+ |
- |
+ |
168.0 ± 9.8 |
168.0 ± 9.8 |
4.4 ± 0.2 |
38.3 ±
4.1 |
Meiothermus sp. PNK-Is4 |
+ |
+ |
- |
29.1 ± 3.5 |
29.1 ± 3.5 |
6.5 ± 1.5 |
4.7 ± 1.7 |
Thermosyntropha lipolytica DSM 11003 |
+ |
+ |
- |
NA$
|
NA |
NA |
/ |
Thermoflexus hugenholtzii JAD2 |
+ |
+ |
+ |
13.9 ± 0.1 |
13.9 ± 0.1 |
0.5 ± 0.1 |
30.6 ±
3.5 |
Petrotoga mexicana DSM 14811 |
- |
+ |
- |
14.1 ± 3.4 |
14.1 ± 3.4 |
1.4 ± 0.3 |
10.0 ±
0.5 |
Kosmotoga pacifica SLHLJ1 |
- |
+ |
- |
9.1 ± 0.8 |
9.1 ± 0.8 |
2.4 ± 0.1 |
3.8 ± 0.8 |
Thermotoga maritima SMB8 |
- |
+ |
- |
10.5 ± 0.3 |
10.5 ± 0.3 |
1.8 ± 0.5 |
5.7 ± 0.1 |
Mutant enzyme |
|
|
|
|
|
|
|
Tk GBE Y233A |
|
|
|
426.2 |
26.3 |
26.3 |
16.2 |
Tm GBE LS |
|
|
|
24.4 ± 3.9 |
6.2 ± 0.1 |
6.2 ± 0.1 |
4.0 ±
0.6 |
Tm GBE LE |
|
|
|
22.6 ± 3.1 |
5.6 ± 0.4 |
5.6 ± 0.4 |
4.0 ±
0.8 |
Tk GBE β sheet deletion |
|
|
|
NA |
NA |
NA |
/ |