Histologic analysis
Soaked the knee joints in 4 % paraformaldehyde for fixation, and then soaked in EDTA to decalcify them. Embedded it in paraffin for slices, and then stained with hematoxylin and eosin (H&E) and tartrate‐resistant acid phosphatase (TRAP). Histology scores were evaluated as published articles[23,24] described, and determined by semiquantitative scoring systems.