Intracellular staining and flow cytometry
As previously described[25,26], splenocytes and PBMCs from CIA mice were washed with PBS. For the analysis of Treg cells, cells were stained with anti-CD4-FITC (BD Bioscience), anti-CD25-PE-CY7 (BD Bioscience) and anti-Foxp3-PE (BD Bioscience). For analysis and gating, we set up auxiliary staining groups including no staining, single staining, isotype staining. Finally, data were acquired on flow cytometry (Beckman FC-500, USA).