Histologic analysis
Soaked the knee joints in 4 % paraformaldehyde for fixation, and then
soaked in EDTA to decalcify them. Embedded it in paraffin for slices,
and then stained with hematoxylin and eosin (H&E) and
tartrateāresistant acid phosphatase (TRAP). Histology scores were
evaluated as published articles[23,24] described,
and determined by semiquantitative scoring systems.