Conserved promiscuous behavior of albumins
We have recently characterized the capacity of BSA to sustain the promiscuous cross aldol reaction involving a set of ketones and benzaldehydes12,14. Here, we have extended the study of the reaction of acetone and p-formylbenzaldehyde to human (HSA), rat (RSA), rabbit (RabSA), and pig (PSA) serum albumins. Besides the sequence divergence of the site involved in such reaction, these five albumins were able to catalyze the reaction (Figure 1). In Figure 2 we represent a sequence alignment of residues involved in the cross aldol condensation following previous characterization14. Following HSA numeration, Lys 199, Arg 218, and Arg 222 are residues involved in this reaction located in the second largest cavity detected by Fpocket in the domain AII as we characterized in previous works14. These residues are highly conserved as we can observe in the alignment of Figure 2.