Albumins cross aldol condensation activity test
HSA, RabSA, RSA, PSA and BSA were tested for the cross aldol condensation between acetone and p -formylbenzaldehyde (using the experimental conditions) following the protocol setted up for bovine serum albumin (BSA) catalysis in previous work14. According to this protocol, a mixture of 54 mg of the assayed albumin was added to a solution of p -formylbenzaldehyde (0.05 mmoles) in acetone-ethanol 1:1 (1 ml). The resulting mixture was shaken at 45 °C and 200 rpm; at different times, aliquots from the biotransformation were taken and after centrifugation of the albumin, submitted to HPLC analysis as reported (10). The conversions to enone (cross aldol condensation product) obtained from each albumin and determined by HPLC are shown in Figure 1. No appreciable conversion was detected in absence of protein, as already observed by us14.