2.2 | DNA extraction and library preparation
High-molecular-weight genomic DNA was extracted from fresh leaves of a
single plant using a DNAsecure Plant Kit (TIANGEN, Beijing, China). For
Nanopore sequencing, a ligation of the sequencing adapters library was
prepared following the manufacturer’s protocol (PromethION, CA). For
Illumina (San Diego, CA) short-read sequencing, libraries were
size-selected for PE150 sequencing. Sequencing libraries with insert
sizes of 350 bp were constructed and sequenced using an Illumina HiSeq X
Ten platform at the Novogene Bioinformatics Institute, Beijing.