2.11 | Drought experiments
The seeds of Pse. libanotica PI 228392 were germinated in a petri
dish lined with a double layer of filter paper. Afterwards, young
seedlings were transplanted to potted monocultures and cultivated in a
greenhouse at a temperature of 20℃ with a light cycle of 16h/8h until
sturdy stage and had a certain number of tillers.
For drought treatments, five month-old potted Pse. libanotica PI
228392 plants of uniform growth were selected to start the experiment.
The experimental groups were not watered for 28 days (every seven days
was taken as a treatment 7d, 14d, 21d, 28d, and each treatment has three
biology repeats), and the control group was watered every 7 days for a
total of 28 days. All leaves were taken at 9-10 am and stored at -80℃.