3.5 | Characterization of the evm.TU.CTG175.54gene related to VCLFA elongation
To characterize the evm.TU.CTG175.54 , we aligned the cDNA in the National Center for Biotechnology Information (https://blast.ncbi.nlm.nih.gov/Blast.cgi, NCBI database), the result showed that it was Kcs5 like and/orKcs6 like in Poaceae species such as T. aestivum ,Ae . tauschii , H. vulgare , Loliumrigidum , Z. mays , Oryza sativa , and Panicum virgatum etc. It shared 73.05% and 77.22% DNA sequence similarity compared with Kcs5 and Kcs6 respectively. Phylogenetic tree based on more than 121 KCS proteins in Poaceae displayed that theevm.TU.CTG175.54 clustered with KCS5 and KCS6, and formed a mixed group (Figure 4). Thus, we speculated that theevm.TU.CTG175.54 might be a new Kcs gene during fatty acid elongation under drought stress in Pse. libanotica and was named as PlKCS5/6.
To investigate the growth of yeast cells exposed to stresses, the yeast cells harboring empty pYES2 (as a control) or pYES2-PlKCS5/6plasmid was evaluated after treated with 2M sorbitol (Figure 5). The results showed that the growth rate of yeast contained empty pYES2 (as a control) or pYES2-PlKCS5/6 had no difference under the treatment of 0M sorbitol. However, the growth of PYES2-PlKCS5/6 plasmid was worse than empty pYES2 under 2M sorbitol treatment. These results demonstrated that expression of PlKCS5/6 gene in yeast related to the abiotic stress treatments.