3.3 | Unique gene families, contract and expanded gene families involved in cuticular wax biosynthesis in Pse. libanotica
To characterizing the St genome, unique gene families and contract and expanded genes familied were analyzed in the St genome. In total, 10,073 shared gene families were identified in published Triticeae species andB. distachyon , and 882 gene families were specific to Pse. libanotica (Figure 2A, 2C; Table S12, 13). Unique gene families inPse. libanotica were mainly involved in ABC transport and cuticular wax biosynthetic pathways (Figure S5). Compared with six Triticeae species, Pse. libanotica contained 3,558 expanded and 7,007 contracted genes (Figure 2C). The expanded gens were enriched in five pathways: cutin, suberine, and wax biosynthesis, sesquiterpenoid and triterpenoid biosynthesis, flavone and flavonol biosynthesis, and benzoxazinoid biosynthesis (Table S14 and S15). In summary, unique gene family and expanded and contract gene family in the St genome are clustered in the cutin, suberine and wax biosynthesis pathway.