Host Aim Input features Achievement Ref
Escherichia coliBL21 Comparing RSM and ANN for optimizing lipase production Glucose, sodium chloride (NaCl), temperature and induction time ANN showed better R2 and adjusted-R2 values, and lower AAD and RMSE values [99]
Sludge Integrating RSM and ANN for improving biohydrogen titer Carbon sources, metal cofactor Fe0, pH, and sludge concentration Developing a robust, cost-effective, and reliable optimization methodology [100]
Kluyveromyces lactis Using ANN coupled with GA for media optimization in hIFN‐γ production Medium components (sorbitol, glycine, Na2HPO4, and MgSO4.7H2O) Achieving maximum productivity in shake flasks level and bioreactor level [101]
Pichia pastoris MLP3 neural network was used to optimize the controller for a novel fed-batch production of A1AT Controller gain and control poles The cell growth and protein titer were improved significantly in comparison to traditional approaches [102]
Rhodotorula glutinis
Establishing ANN and SVM models for microbial lipid production
Biomass, lipid yield, and COD removal rate
SVM performed better than ANN for small samples Fermentation parameters were optimized by integration of SVM and GA
[103]
Sludge Developing a hybrid model for optimizing CBP production using LS-SVM and OED Corn stalk weight, ultrasonic duration time, pretreatment time, and dual-frequency ultrasound The model increases CBP 14.13% more than pure OED [104]
Escherichia coli Using random forest and ANN for biomass and recombinant protein modeling in a fed-batch process Temperature, induction strength, growth rate, soluble/insoluble product formation The accuracy reached about ±4% for dry cell mass and ±12% for protein concentration [106]
saccharomyces cerevisiae Employing GPR for prediction of S. cerevisiae biomass concentration Molasses feed rate Experimentally validation results showed high accuracy [107]
Penicillium brevicompactum Establishing a k-nearest-neighbor model to optimize different independent factors in MPA production Ultrasound power, irradiation duration, treatment frequency and duty cycle 1.64-fold improvement observed in MPA production [108]