Parameter Aim Achievement Ref
Culture medium Combining the FBA with Plackett Burman (PB) for a recombinant Escherichia coli Identifying the key amino acids and carbon sources for successful improvement in siderophore production [67]
Culture medium Using the FVSEOF algorithm to design feeding strategies for CHO cells Suggesting threonine and arachidonate as supplements by the metabolic model and a two-fold increase in antibody production [68]
Culture medium Designing a strategy for amino acid supplementation by a stoichiometric model Enhancing the production of interleukin-2 by adding a mixture of leucine, aspartic acid, and glycine [69]
Culture medium
Performing FBA for C. sporogenes NCIM on glucose, glycerol & their mixture
Glucose and glucose-glycerol mixture improve the growth and the butyric acid production. Glycerol reduces the growth and improves ethanol yield
[71]
pH MFA used for Bacillus licheniformis to study the effect of pH on metabolic flux distribution Proposing a pH-controlled strategy to improve β-lactamase production [8]
pH FBA used to gain mechanistic insight into the question of how pH affects lactate metabolism for a mammalian cell Lowering the pH increases lactate consumption, antibody production and makes the cell more energy-efficient [72]
Temperature FBA implemented to study the effect of mild hypothermia conditions on the CHO cell behaviors Formation of premature glycosylated antibodies during the stationary phase at 32 °C [73]