Immunofluorescence assay
For CLSM imaging to confirm biofilm components or record the live/dead
ratio of the B. pseudomallei and B. thailandensisbiofilms, inoculated 96 well were gently washed and molecular dyes: Film
Tracer biofilm viability kit (Invitrogen L10316), DAPI stain (KPL,
71-03-00), Sypro ruby biofilm matrix stain (Invitrogen, F10318), and the
Wheat germ agglutinin fluorescein conjugate (Invitrogen, W834) were used
to stain wells according to each manufacturer’s protocol. Wells were
washed with sterile water and imaged immediately using a Nikon Eclipse
90i microscope.