Bacteria strains
Five B. pseudomallei strains (1026b [36], DD503 [39],
Bp1026b Δasd [37], Bp82 [38], and JW270 [40])
and B. thailandensis E264 [35] were used for this study.
Strains were either cultured on LB agar (1026b, JW270 and E264), LB agar
with 100 µg/ml diaminopimelic acid (DAP) (1026b Δasd ) or LB
supplemented with 100 µg/ml adenine and 5µg/ml thiamine (Bp82).
Bacterial colonies were inoculated in 3ml of LB broth, with or without
the corresponding supplement, and grown overnight in a 37°C incubator
with shaking (250 rpm) or without agitation. Bacteria were serially
diluted, plated to determine bacteria count, and used for experiments.
All manipulations with B. pseudomallei 1026b and DD503 were
carried out in a class II microbiological safety cabinet located in a
designated biosafety level 3 (BSL-3) laboratory.