Immunofluorescence assay
For CLSM imaging to confirm biofilm components or record the live/dead ratio of the B. pseudomallei and B. thailandensisbiofilms, inoculated 96 well were gently washed and molecular dyes: Film Tracer biofilm viability kit (Invitrogen L10316), DAPI stain (KPL, 71-03-00), Sypro ruby biofilm matrix stain (Invitrogen, F10318), and the Wheat germ agglutinin fluorescein conjugate (Invitrogen, W834) were used to stain wells according to each manufacturer’s protocol. Wells were washed with sterile water and imaged immediately using a Nikon Eclipse 90i microscope.