eDNA concentration
Using a 96-well plate, triplicates of 25 µl of extractant was added to 175 µl of 0.1% propidium iodide dissolved in water. The reaction was incubated with gentle agitation in the dark, at RT for 10 mins to mix. Wells were excited at 535nm and florescence read at 620nm. 200 µl of 0.1% propidium iodide was used as blank.