Validating single guide RNA for MSTN in bovine fibroblast cells
In bovine fibroblast cells, the selected sgRNA caused a mutation on the target site. The mutation on MSTN was detected by T7E1 assay (data not shown) and sanger sequencing. Though the sanger sequencing results, various types of mutation pattern were observed (Fig 1B). These results show that the selected sgRNA worked well on the MSTNtarget site.