Statistical analysis
All data are presented as mean ± SEM. Newman–Keuls Multiple Comparison
Test and Tukey’s test in a one-way ANOVA were used to evaluate
statistically evaluate differences in embryo development, frequency of
mutagenesis, and mRNA expression level. A value of P<0.05 was
considered statistically significant. All analyses were performed using
the GraphPad Prism program (version 5.01).