Figure 4. Analysis of cellular response to IL-12 and IL-23 of
TYK2- deficient patients.
(A and B) Total STAT4 and tyrosine-phosphorylated STAT4 (pSTAT4) protein
level were analyzed by western blot in P2, P3 and healthy control. The
patients’ PBMC were stimulated with IL-12 (100ng/μL) for 15 min.
(C and D) Total STAT3 and pSTAT3 protein level were analyzed by western
blot in P2, P3 and healthy control. The patients’ PBMC were stimulated
with IL-23 (100ng/μL) for 15 min.
(E) Flow cytometry analysis showing intracellular IFN-γ production in
PBMCs after stimulation in the absence or presence of PMA (50ng/ml) and
Ionomycin (500ng/ml) for 5 hrs or BCG or BCG and 100 ng/ml IL-12 for 72
hrs. An anti-CD3 Ab was used to identify CD3+ T cells
in P3 and healthy control.
Representative images from 2-3
independent experiments.