Figure 4. Analysis of cellular response to IL-12 and IL-23 of TYK2- deficient patients.
(A and B) Total STAT4 and tyrosine-phosphorylated STAT4 (pSTAT4) protein level were analyzed by western blot in P2, P3 and healthy control. The patients’ PBMC were stimulated with IL-12 (100ng/μL) for 15 min.
(C and D) Total STAT3 and pSTAT3 protein level were analyzed by western blot in P2, P3 and healthy control. The patients’ PBMC were stimulated with IL-23 (100ng/μL) for 15 min.
(E) Flow cytometry analysis showing intracellular IFN-γ production in PBMCs after stimulation in the absence or presence of PMA (50ng/ml) and Ionomycin (500ng/ml) for 5 hrs or BCG or BCG and 100 ng/ml IL-12 for 72 hrs. An anti-CD3 Ab was used to identify CD3+ T cells in P3 and healthy control.
Representative images from 2-3 independent experiments.