Figure 2. Analysis of cellular response to type I&II IFNs in TYK2-deficient patients.
(A-B) Total STAT1, STAT3 and tyrosine-phosphorylated STAT1(pSTAT1) and STAT 3 (pSTAT3) protein level were analyzed by western blot in P1, P2 and healthy control. The patients’ PBMC were stimulated with IFN-α (100ng/μL), IFN-β (500IU/mL) or IFN-γ (100ng/μL) for 15 min. Representative images from 2-3 independent experiments.
(C-D) Analysis of type I IFN-inducible gene induction in PBMCs from healthy control and P1 and P2. Cells were stimulated with IFN-α (100ng/μL), IFN-β (500IU/mL) or without IFN for 4 h. Data are derived from 2-3 independent experiments.