Figure 5. Characterization of T-cell and B-cell subsets in TYK2- deficient patients.
(A and B) Gating strategy for identifying Th1/2/17 (A), and Th1/2/17-like cells (B) by flow cytometry in healthy control and patients.
(C-F) Percentage of Th1/2/17, and Th1/2/17-like cells in P1, P2, P3 and healthy control (n=6).
(G- J) Gating strategy for identifying Tfh (G), Tfh expressing PD-1 (H), Tfr (I) and Treg (J) by flow cytometry in healthy control and patients.
(K, L and N) Percentage of Tfh, Tfh expressing PD-1, Tfr and Treg in P1, P2, P3 and healthy control (n=6).
(M) Gating strategy for identifying IgMhi (M), MZ-like (M) and Sm B cells (M) by flow cytometry in healthy control and patients.
(O and P) The frequency of B cell subsets in P2 (O) and healthy control (n=5), P3 (P) and healthy control (n=6).