Real-Time Quantitative PCR
Total RNA was extracted from peripheral blood with the Blood Total RNA
Miniprep Kit (Axygen). The cDNA was transcribed by the Transcriptor cDNA
Synth. Kit (Roche). Levels of targeted mRNA were determined using a
Bio-rad CFX96 Touch (BIO-RAD) machine. The results of each gene were
determined with the 2−ΔΔCt method, and data was expressed as fold
induction. Primers were listed in Supplementary table 2.