Figure 4. Cytokine production and transcription factor
expression in Tr17 cells
a) Lymphocytes in draining lymph node were harvested from C57BL/6-Foxp3
GFP reporter mice 10 days after first CII immunization.
CD4+GFP+ Treg cells and
CD4+GFP- T cells were isolated and
stimulated with TCR stimulation in vitro . The expression of IL-10
and IL-17 in RORγt+GFP+ Tr17 cells
was analyzed using flow cytometry and compared with that in
RORγt-GFP+ cells and
RORγt+GFP- cells. b) At 10 days
after CII immunization, Blimp-1, Foxp3, and RORγt expression in Tr17
cells, RORγt-Treg cells, and
RORγt+non-Treg cells in lymph node were analyzed using
flow cytometry.