2.5.3 Swelling kinetics study
The swelling behavior of the control SA/Gel as well as the fabricated
A-SA-Gel hydrogel samples was analyzed by immersing the samples in
Roswell Park Memorial Institute (RPMI) 1640 culture medium and phosphate
buffered saline (PBS) at pH 7.4 at 37 °C for 3 h. The swelling percent
(%) was calculated according to the following equation:
Swelling (%) = [(Wt – Wo)/
Wo ] × 100%
For which, the samples were dried at 37 °C and the dry weight
(Wo ) of the same was captured. Then, the samples
were immersed in culture medium and PBS. The swollen weight
(Wt ) of the samples was measured every 15 min
after removing excess solution from the surface of the samples with
lint-free filter paper, until no further change in weight. All samples
were analyzed in triplicate.