Fig. 1 Ploidy and genotype identification of parent LM50 and its anther plant GM15. (a) The regenerated individual (GM15) from anther ofP. tomentosa male clone LM50, (b) Ploidy detection of LM50 and GM15 by flow cytometry, (c) Chromosome counting of LM50 and GM15, (d) Genotype identification of LM50 and GM15 by PCR using allele-specific primers derived from 19 chromosomes. (M) marker DL2000, (P) Parent (male clone LM50), (A1) Anther plant GM15. (e) Genome sizes of LM50 and GM15 estimated by K-mer analysis.
Figure 2