Material and Methods
Schizochytrium sp. micro algae species was used as the study
material. Spray-dried samples were obtained from Marin Biotechnology
Products and Food Industry (Aydın/Turkey).
In order to increase the lipid yield of this algae, 4 different cell
disruption methods were tried; treatment with HCl in the reflux, osmotic
shock, enzyme and ultrasonic homogenizer. After each cell disruption
method, classical method of Bligh and Dyer (1959) and Soxhlet extraction
methods of AOAC (2006) were applied for total crude lipid. Bligh and
Dyer (1959) and AOAC (2006) Soxhlet methods applied alone were accepted
as control groups. The groups were coded as follows; Bligh and Dyer and
Soxhlet groups; control (BDC and SC), osmotic shock (BDOS and SOS),
enzyme (BDE and SE), HCl (BDHCl and SHCl), ultrasonic homogenizer (BDUH
and SUH); respectively. In each method, 2 g of dry algae samples were
used. Each analysis was carried out in three parallel.