Conclusions
In the study of total crude lipid methods combined with cell disruption
applications to increase lipid yield of Schizochytrium sp., Bligh
and Dyer method was found to be more applicable than Soxhlet. According
to the results, Schizochytrium sp. is the best source of DHA
essential fatty acid. Higher amount of DHA that is necessary for human
health, is advantageous to be used in many applications such as food,
feed and cosmetics. Total lipit and fatty acids were found higher than
Soxhlet in Bligh and Dyer groups. Soxhlet method can be good at lipid
extraction, however damages fatty acids. Lipid health indices such as
n-6/n-3, PUFA/SFA, AI, TI and HH were almost favorable. To get higher
lipid yield and quality from algal cells, a suitable cell disruption
method is required. The enzyme application combined with Bligh and Dyer
method is thought to give the most efficient result, with being
high-efficiency, environmentally friendly, non-toxic and industrial
scale feasibility. As a recommendation, combined cell disruption methods
can be used in future studies.