HCl extraction
In the application of HCl as solvent, the algae: HCl (3 N) (1:40) was boiled at reflux for 1 hour (acid hydrolysis step). The sample was passed through filter paper and washed with 500 mL of water and filtered. After filtering, the filter paper was dried for 1 hour in an oven at 103 °C (AOAC, 2005). The dried samples were continued with Bligh and Dyer and Soxhlet methods.