Figure 2: Equilibrium unfolding of HIV-1-PR1-95. Top:
mean residue ellipticity at 230 nm of a 15 µM HIV1-
PR1-95 in 20 mM sodium phosphate, pH 6, measured in the
presence of increasing concentrations of urea (left), GdmCl (middle) and
acetic acid (right) at 25 °C. Bottom: wavelength of maximum fluorescence
emission of 4 µM mHIV-1-PR1-95 sample 20 mM sodium
phosphate buffer, pH 6, measured in the presence of increasing
concentrations of urea, GdmCl or acetic acid, at 25 °C (excitation
wavelength: 295 nm).