2.6 Preparation of DNA, PCR amplification of 16S rRNA genes, and
Sanger sequencing
For the phylogenetic analyses of bacterial isolates, genomic DNA was
extracted from the cells of the isolates and purified by the method of
Saito and Miura (1963). PCR amplification of 16S rRNA genes with primers
27F and 1492R, and sequencing of the PCR products were performed as
described previously (Iino et al. , 2015a).