2.9 Animal model
All animal experiments were approved by the Animal Care and Use
Committee of Shandong University, where the experiments were conducted.
All animals were housed in a temperature-controlled room (22 ± 2 °C)
with a light/dark cycle of 12 h. Male C57BL/6J mice at the age of 8
weeks were randomly divided into four groups (n = 8): the control group
(Veh), which received an equal amount of 0.5% sodium
carboxymethylcellulose (CMC-Na) solution; LPS-treated group (10 mg/kg
LPS), which received LPS (10 mg/kg) only via intraperitoneal injection;
270-treated groups (25 mg/kg and 50 mg/kg, LPS-270-(25) and
LPS-270-(80)), which received 270 at different doses (25, 50 mg/kg) via
intragastric administration before LPS stimulation. All mice were
consecutively given 270 or CMC-Na for 14 days, at the last day, all mice
(except for those in the control group) were treated by intraperitoneal
injection with 10 mg/kg body weight LPS to induce septic acute kidney
and lung injury. After 24 h of LPS stimulation, all experimental mice
were sacrificed; fasting blood was collected and used for cytokine
determination, partial kidney and lung tissues were fixed and used for
haematoxylin and eosin (HE) staining, remaining tissues were carefully
harvested, immediately frozen in liquid N2, and stored
at −80 °C for subsequent analysis.