2.10 Biochemical tests and detection of cytokines
The concentrations of TNF-α, IL-1β, IL-6 and MCP 1 in mice plasma and
BMDMs supernatants were detected using ELISA kits, according to the
manufacturer’s instructions. To evaluate kidney function, urea nitrogen
(BUN) and creatinine (Crea) levels in mice plasma were estimated using
commercially available kits (Xinjiankangcheng), and performed following
the manufacturer’s protocol.