2.5 RAW 264.7 cells culture and treatment
RAW 264.7 cells were purchased from Cell Bank, and cultured in HG-DMEM
medium containing 10% FBS. All cells were seeded into 24-well cell
culture plates at a concentration of approximately
3x105 cells per well, maintained in a humidified
atmosphere of 5% CO2 at 37 ˚C, and allowed to grow for
24 h. LPS was used to trigger inflammatory responses. RAW 264.7
macrophages were treated with LPS alone (100 ng/mL) or LPS with 270 for
24 h, harvested cells were used for RNA extraction. RAW 264.7 cells were
pretreated with 270 for 4 h before LPS (100 ng/mL) treatment for 30 min,
and cells lysates were obtained for western blot.