2.9 Animal model
All animal experiments were approved by the Animal Care and Use Committee of Shandong University, where the experiments were conducted. All animals were housed in a temperature-controlled room (22 ± 2 °C) with a light/dark cycle of 12 h. Male C57BL/6J mice at the age of 8 weeks were randomly divided into four groups (n = 8): the control group (Veh), which received an equal amount of 0.5% sodium carboxymethylcellulose (CMC-Na) solution; LPS-treated group (10 mg/kg LPS), which received LPS (10 mg/kg) only via intraperitoneal injection; 270-treated groups (25 mg/kg and 50 mg/kg, LPS-270-(25) and LPS-270-(80)), which received 270 at different doses (25, 50 mg/kg) via intragastric administration before LPS stimulation. All mice were consecutively given 270 or CMC-Na for 14 days, at the last day, all mice (except for those in the control group) were treated by intraperitoneal injection with 10 mg/kg body weight LPS to induce septic acute kidney and lung injury. After 24 h of LPS stimulation, all experimental mice were sacrificed; fasting blood was collected and used for cytokine determination, partial kidney and lung tissues were fixed and used for haematoxylin and eosin (HE) staining, remaining tissues were carefully harvested, immediately frozen in liquid N2, and stored at −80 °C for subsequent analysis.