2.12 Determination of MPO and MDA
About 30 mg of left upper kidney and lung tissues were homogenated in
cold saline solution at a ratio of 1:10 (weight:volume). Myeloperoxidase
(MPO) activity is a biochemical marker for the infiltration of
neutrophils and macrophages. Malondialdehyde (MDA) is an indicator of
oxidant stress (Jiang et al. ,
2019). MPO activity and MDA content were measured using a commercially
available kit following manufacturer’s instructions (Nanjing Jiancheng
Bioengineering Institute). Data were calculated as the absorbance after
normalization by the protein content.