Figure 3. Compound 270 suppresses the LPS-induced inflammatory response in RAW 264.7 macrophages.
(A) RAW 264.7 cells were pre-incubated with 270 for 4 h and then treated with LPS (100 ng/ml) for 30 min. Total cell lysates were subjected to western blot. (B) The protein levels shown in (A) were quantified by densitometry analysis. (C) RAW 264.7 cells were treated with LPS (100 ng/ml) alone or together with 270 for 24 h. Relative mRNA level of each pro-inflammatory gene was analyzed by use of RT-PCR and normalized by GAPDH. The data are presented as the means ± SEM. All experiments were performed in three replicates. Two-tailed Student’s t-test. *P < 0.05, **P <0.01, ***P < 0.001 versus LPS group.
Figure 4