Laboratorial methods
Respiratory samples (nasopharynx swab and/or tracheal aspirates) were submitted to molecular analysis at the Molecular Biology Laboratory HCFMUSP: Fast-track Diagnostics® (Panel 21), detects 21 respiratory pathogens: adenovirus, bocavirus, coronavirus (229E; HKU1; NL63; OC43), human rhinovirus/enterovirus, influenza virus A (H1N1, H3N2, Influenza A H1N1/2009), influenza virus B, Influenza virus C, metapneumovirus A e B, Mycoplasma pneumoniae, parainfluenza virus (1-4), parechovirus, respiratory syncytial virus (RSV) A and B.(5) RT-PCR for SARS-CoV-2 analysis was performed, according to the Charité University protocol.(6)
Serology was performed at the HCFMUSP Immunology Laboratory by imunocromatographic test (SARS-CoV-2 antibody test® WONDFO) or by enzyme linked immunosorbent assay (LIAISON® XL | DiaSorin).(4)