Chain(s) Mutation(s) Average distance (Å) Individual ΔΔGBinding (Kcal/mol) Predicted ΔΔGBinding (Kcal/mol) Effect
H
L219A V225A L233A
9.75
-1.39 -0.22 -0.43
-1.15
Decreasing affinity
H
K234A L238A
6.16
-2.15 -1.09
-2.88
Decreasing affinity
M
L94A L163E
4.84
-1.61 -1.19
-2.09
Decreasing affinity