Model quality evaluation
Following the structural refinement of the three models with
GalaxyRefine, we proceeded with the protocol for the quality evaluation
of each model. Using the ProSA-web, the z-score for each model was
obtained (Figure 2). TheĀ z -score is an indication of the overall
model quality. hs CENP-H, hs CENP-K and hs CENP-I
produced individual z-scores of -3.17, -4.66 and -7.38 respectively,
indicating that all the three models fall within the quality range of
the nuclear magnetic resonance (NMR) as shown in Figure 2.