Model quality evaluation
Following the structural refinement of the three models with GalaxyRefine, we proceeded with the protocol for the quality evaluation of each model. Using the ProSA-web, the z-score for each model was obtained (Figure 2). TheĀ z -score is an indication of the overall model quality. hs CENP-H, hs CENP-K and hs CENP-I produced individual z-scores of -3.17, -4.66 and -7.38 respectively, indicating that all the three models fall within the quality range of the nuclear magnetic resonance (NMR) as shown in Figure 2.