2.6 Significance analysis of epitope amino acids
The amino acid descriptor 3z scales (z1 descriptor represents
hydrophilicity, z2 represents the bulk, and z3 represents electricity
properties) were used as the X variable and the T cell epitope
allergenicity (yes/no) was set as the Y variable, [26]. Each T cell
epitope had 15 aa and the number of variables for each soybean allergen
was 45 (3*15), expressed a,s p1z1-p15z3. The discriminant analysis was
performed with the XLSTAT software (Trial version, Addinsoft, New York,
NY) to confirm the accuracy of the amino acid descriptor 3z scales. then
the significance analysis of T cell epitope amino acids was carried out
by using the logistic regression and random forests via the Easy Fit
function of XLSTAT software.