2.4 Allergenicity evaluation
The amino acid sequences of the T cell epitopes were submitted to the online tool AllerTop 2.0 (https://www.ddg-pharmfac.net/AllerTOP/), and the amino acid properties, such as hydrophobicity, size, helix-forming propensity, relative abundance, and β-strand forming propensity, were applied for the allergenicity prediction [23, 24].