2.6 Significance analysis of epitope amino acids
The amino acid descriptor 3z scales (z1 descriptor represents hydrophilicity, z2 represents the bulk, and z3 represents electricity properties) were used as the X variable and the T cell epitope allergenicity (yes/no) was set as the Y variable, [26]. Each T cell epitope had 15 aa and the number of variables for each soybean allergen was 45 (3*15), expressed a,s p1z1-p15z3. The discriminant analysis was performed with the XLSTAT software (Trial version, Addinsoft, New York, NY) to confirm the accuracy of the amino acid descriptor 3z scales. then the significance analysis of T cell epitope amino acids was carried out by using the logistic regression and random forests via the Easy Fit function of XLSTAT software.