2.3 Histological assay
The adenoid tissue specimens were fixed in 4% paraformaldehyde,
embedded in paraffin, and then cut into 3 mm sections. The tissue
sections were dried overnight at 37℃ and stained with hematoxylin &
eosin to count the number of eosinophils. Sections were coded and
randomly analyzed by two blinded researchers using an electron
microscope (Olympus, Beijing, China). After antigen resuscitation,
adenoid tissue sections were incubated with 3%
H2O2 for 5 min, and then with 10%
normal goat serum for 10 min. Next, the tissue specimens were incubated
with anti-CysLTR1 (1:100; PA2310; Innovagen, Lund, SWEDEN), anti-CGR-α
(1:50; ab3580; Abcam, Shanghai, China), and anti-CGR-β (1:50; ab3581;
Abcam, Shanghai, China) antibodies at 37℃ for 1 h. Finally, the tissue
specimens were stained using diaminobenzene (1 mg/mL, Sigma).