2.2 Life history traits
Newly emerged adult females of the first lab generation were weighed
before feeding and reared individually thereafter. Three days after
eclosion, each female was paired with a male from the same population of
about the same age and allowed to mate for 24 hours before the male was
removed. Each 0.24 L container with a female was checked daily for
oviposition. All eggs that were laid on the first day of oviposition
were counted to provide a measure of early fecundity and the age at
first reproduction in days was recorded. If an individual had not
oviposited within 10 days of emergence, they were counted as non-layers
(Lewis et al. 2003).