Functional assay
For CD107a expression analysis, TILs were seeded in a 96-well plate as 1e5/well, and three duplicated wells were set for each group. TransAct (Miltenyi) was added to stimulate TILs overnight. Then, CD107a was analyzed by flow cytometry on the next day. For cytokine secretion and cytotoxicity analysis, HeLa cells labeled with CFSE were seeded in a 96-well plate as 5e4/well with 1.5e5/well TILs. The supernatant was collected at 18hrs and cytokine production was analyzed by CBA (BD). Then, DAPI was added into the co-culture system to analyze the tumor killing by detecting dead cells with flow cytometry.