TIL preparation
Primary tumors or lymph node metastases were separated into 1-20mm3
fragments and placed in a Grex or gas permissible bag. In preREP phase,
the tissues were expanded with timely replenished or replaced medium for
about two weeks. Culture medium contained 3000IU/mL rhIL-2 (T&L
Biotechnology). In the following REP phase, TILs were stimulated with
anti-human CD3 antibody (OKT3, T&L Biotechnology) in the presence of
irradiated feeder cells and then expanded for about two weeks.