Figure 5. uCDR3 diversity characterization. (A). Unique CDR3 comparison between PBMC and TIL. Paired t test P-value was 0.00133.(B). Unique CDR3 counts in each sample. Mean uCDR3 counts of PBMC, TIL and total samples were shown in colored dash lines as indicated. (C). Percentage stacked barplot by sample. Colored bars indicate uCDR3 in different rank ordered by uCDR3 clone abundance. For example, red bar shows the total percentage of the Top10 ranked uCDR3 in each sample. (D). Shannon Entropy comparison between PBMC and TIL. Paired t test P-value was 0.0004. (E). Shannon Entropy in each sample. Mean Shannon Entropy of PBMC, TIL, and total samples were shown in colored dash lines as indicated.