Figure 7.Functional characterization
of CC and VC TILs. TILs were assessed for cytokine secretion and tumor
killing capacity after rapid expansion. (A). TILs were
re-stimulated by antibodies and CD107a expression was analyzed by flow
cytometry. (B-C). IFN-γ and TNF-α were detected by CBA in the
supernatants of TIL after co-culture with HeLa cells line with an
effector to target ratio of 3:1 overnight. Mean of IFN-γ and TNF-α level
(pg/ml) is shown above in each bar. (D). HeLa cells were
labeled with CFSE before co-culturing with TILs. Cell lysis was
monitored by flow cytometry after 18hs. Tumor killing capacity is shown
by the percentage of tumor cell lysis in the co-culture. (E).Representative flow chart for CD107a expressed.