Counts and reads fraction distribution of TCR clonotypes
A total of 107732 clonotypes were
identified in all TIL samples. Detection times of each clonotypes in TIL
samples were summarized in Figure 6A. Most clonotypes (98.467%) were
detected in only one TIL sample. Only 1.53% clonotypes were found in
more than one TIL sample and 0.01% were found in 4 or more samples,
suggesting that the repertoire of TIL TCR were patient-specific (Figure
6A). Reads of most shared clonotypes represented low fraction, with
77.8% of clonotypes accounting for less than 0.001% reads fraction and
18.2% clonotypes accounting for 0.001%-0.01% (Figure 6B).