Expansion of TIL from vulvar and cervical tumors
VC TILs could be successfully expanded using the same procedure as that for CC TILs. Briefly, after the acquisition and transportation of tumor surgical specimens to our manufacturing site, TIL cells were collected from the tumor fragments, cultured in a previous rapid expansion phase (preREP), and then amplified in a rapid expansion phase (REP). Expanded TIL cells were then cryopreserved and transported to the clinical center (Figure 1A). The average cell number in preREP from the four batches of VC TILs is greater than 2×107 (Figure 1B), and the average expansion fold in REP is greater than 1000 (Figure 1C), which is higher than that of CC TILs. In general, VC TILs can be successfully cultured and amplified in vitro.