Figure 4. Stemness and memory phenotype analysis of CC and VC TILs. TILs were analyzed for the expression of indicated markers using flow cytometry after 9-14 days of rapid expansion. (A-B). The percentage of PD1 or TCF1 expression in TILs of each batch is shown. (C-D). The percentage of different T cell memory phenotypes is shown. Naïve, central memory, effector memory and effector memory RA T cells are defined by CD45RA+CCR7+, CD45RA-CCR7+, CD45RA-CCR7- and CD45RA+CCR7-, respectively.(E). The percentage of CD103 and CD39 expression in TILs of each batch is shown. (F). Representative flow chart for each marker expressed.