Figure 4. Stemness
and memory phenotype analysis of CC and VC TILs. TILs were analyzed for
the expression of indicated markers using flow cytometry after 9-14 days
of rapid expansion. (A-B). The percentage of PD1 or TCF1
expression in TILs of each batch is shown. (C-D). The
percentage of different T cell memory phenotypes is shown. Naïve,
central memory, effector memory and effector memory RA T cells are
defined by CD45RA+CCR7+,
CD45RA-CCR7+,
CD45RA-CCR7- and
CD45RA+CCR7-, respectively.(E). The percentage of CD103 and CD39 expression in TILs of
each batch is shown. (F). Representative flow chart for each
marker expressed.