Expansion of TIL from vulvar and cervical tumors
VC TILs could be successfully expanded using the same procedure as that
for CC TILs. Briefly, after the acquisition and transportation of tumor
surgical specimens to our manufacturing site, TIL cells were collected
from the tumor fragments, cultured in a previous rapid expansion phase
(preREP), and then amplified in a rapid expansion phase (REP). Expanded
TIL cells were then cryopreserved and transported to the clinical center
(Figure 1A). The average cell number in preREP from the four batches of
VC TILs is greater than 2×107 (Figure 1B), and the average expansion
fold in REP is greater than 1000 (Figure 1C), which is higher than that
of CC TILs. In general, VC TILs can be successfully cultured and
amplified in vitro.