Figure 3.Profiling of CC and VC TILs at the end of REP. (A-B). At the end of REP culture, TILs were collected from each batch and the percentage of CD3+ T cells, CD4+ T cells, CD8+ T cells, and CD3-CD56+ NK cells was measured by flow cytometry. (C). CD28 expression on CD4+ and CD8+ T cells was measured by flow cytometry. (D). TIM3 and LAG3 expression on CD4+ and CD8+ T cells was measured by flow cytometry. (E). Treg ratio in TILs was analyzed with CD25 and Foxp3 staining by flow cytometry. (F). Representative flow chart for each marker expressed.