2.5 Mosquitoes
The Poza Rica Ae. aegypti strain was colonized in 2012 from
collections in the state of Veracruz, Mexico(Vera-Maloof,
Saavedra-Rodriguez, Elizondo-Quiroga, Lozano-Fuentes, & Black IV,
2015). The Cx. tarsalis Kern National Wildlife Refuge (KNWR)
colony(Oviedo, Romoser, James, Mahmood, & Reisen, 2011), established in
1952, was obtained from the Centers for Disease Control and Prevention
(Fort Collins, CO). Mosquito colonies were maintained at
24-26oC (Culex ) or 28oC
(Aedes ) at 12:12 light:dark cycle; adults were fed water and
sucrose ad libitum . Larvae were reared on ground TetraMin fish
food (http://www.tetra-fish.com/).