2.12 Data Analysis
P was determined by calculating the proportion of viral RNA-positive mosquito bodies for the combined total number of mosquito RNA samples. Dissemination was determined by calculating the proportion of legs/wings RNA samples with detectable RVFV RNA against the total number of mosquitoes exposed. Transmission was determined by calculating the proportion of saliva RNA samples that were RVFV-RNA positive against the total number of mosquitoes exposed. Percent of saliva expectorants containing infectious virus were also calculated by determining the proportion of saliva samples producing detectable CPE by plaque assay among the total number of individuals tested. The percentage of RVFV-infected mosquitoes after feeding on inoculated goats were determined by calculating plaque positive mosquito bodies per total number of mosquitoes assayed. RVFV growth curve titers were analyzed by calculating the highest dilution containing countable plaques and multiplying that by the dilution factor to obtain log10PFU/ml.
All graphing and statistical tests were performed in Prism Graphpad (version 8, https://www.graphpad.com/). χ2 contingency tests were used to calculate dissemination and transmission rates. Two way ANOVA (analysis of variance) with Geisser-Greenhouse correction was used to determine differences in viral growth kinetics. One way ANOVA was used to determine differences in bloodmeal titers.