2.7 RNA extractions
RNA extractions of individual 50µl sample aliquots were performed using
the Applied Biosystems MagMax-96 Viral RNA extraction kit (AMB1836-5,
Thermofisher) following the manufacturer’s protocol for manual
extraction methods (MAN0017826). Linear polyacrylamide was used as a
carrier in place of carrier RNA. Reactions were eluted into 50µl elution
buffer and stored in 96-well plates at -80oC.