Comparison of gene-by-gene level patterns
At a gene-by-gene level, there was no clear relationship among average
FST and \({\overset{\overline{}}{H}}_{E}\) for
orthologous genes for any species pair (Fig. 3). Average
FST per gene was weakly correlated among all species
pairs, with tubesnouts and ninespines having a negative albeit
non-significant correlation (Table 2). A lack of similarity was also
observed with \({\overset{\overline{}}{H}}_{E}\) scores, with a slightly
stronger negative correlation between threespines and ninespines (Table
2). Additionally, pairwise comparisons between populations showed less
similarity in \({\overset{\overline{}}{H}}_{E}\) for among-species
comparisons (ρ < 0.2) than within-species comparisons (ρ
> 0.4; Fig. 3B). No clear visual pattern exists in\({\overset{\overline{}}{H}}_{E}\) (Fig. 3A) or FST(Fig. 3C), with the exception of a flattening of\({\overset{\overline{}}{H}}_{E}\) and elongation of FSTtowards the ninespine axes. Overall, these patterns show broad-scale
similarity between threespines and tubesnouts, which does not extend to
the local gene level, or overlap with ninespines.