Multiple sequence alignment
Multiple alignment of Hsp60 amino acid sequences was performed using the ClustalOmega software26 and the BioPython 1.68 library27. Multiple alignment of Hsp60 nucleotide sequences was performed using the MUSCLE program28 and the MEGA-CC software29. The nucleotide sequences were aligned using the MUSCLE Codons option. Here, all codons in the sequences have been converted to amino acids using the standard genetic code. The obtained amino acid sequences were aligned with subsequent reverse translation of amino acids into codons. Note that this option is valid for encoding nucleotide sequences in multiples of three and without reading frame displacement.