Isolation of isoprenoids
Unless indicated otherwise, entire 3-week-old seedlings were used for
the isolation of all isoprenoid compounds. To elucidate the correlation
between polyisoprenoid content vs. CPT3 transcript level, the
Arabidopsis seedlings, leaves, and flowers were used. For qualitative
and quantitative analysis of isoprenoids, either internal (Prens, Dols,
and phytosterols) or external (plastoquinone and tocopherol) standards
were employed. For quantitative analysis of Prens, Dols, phytosterols,
plastoquinone, and tocopherols signals corresponding to compounds of
well-characterized structure were taken into consideration, exclusively.
Prens, Dols, phytosterols, plastoquinone, tocopherols, carotenoids and
chlorophylls were isolated and quantified using standard methods – for
details see Supplementary Methods.