Phenotypic variation in isoprenoid content in the AI-RIL mapping population
Next, the seven isoprenoid compounds described above (carotenoids, chlorophylls, Dols, phytosterols, plastoquinone, Prens, and tocopherols) were quantified in 146 lines of the EstC mapping population and its parental lines (Col-0 and Est-1). The profiles of analyzed isoprenoids were similar to those described above for different accessions, while the level of particular compounds varied among lines of the mapping population (shown in details in Figure 1B, Figure S3, and Table 1). The range of the content of Prens (Figure 1B), Dols (Figure 1B) and other compounds (Figure S3) was broader than that observed for both parental lines, which might suggest that several loci within the EstC population contribute to this phenomenon and it may be explained by the presence of transgressive segregation.