Isolation of isoprenoids
Unless indicated otherwise, entire 3-week-old seedlings were used for the isolation of all isoprenoid compounds. To elucidate the correlation between polyisoprenoid content vs. CPT3 transcript level, the Arabidopsis seedlings, leaves, and flowers were used. For qualitative and quantitative analysis of isoprenoids, either internal (Prens, Dols, and phytosterols) or external (plastoquinone and tocopherol) standards were employed. For quantitative analysis of Prens, Dols, phytosterols, plastoquinone, and tocopherols signals corresponding to compounds of well-characterized structure were taken into consideration, exclusively.
Prens, Dols, phytosterols, plastoquinone, tocopherols, carotenoids and chlorophylls were isolated and quantified using standard methods – for details see Supplementary Methods.