Complementation of the yeast rer2Δ mutant
To express CPT3 and LEW1 in Saccharomyces cerevisiae mutant cells (rer2 Δ mutant: rer2 ::kanMX4ade2-101 ura3-52 his3-200 lys2-801 ), coding sequences of CPT3 and LEW1 (AT1G11755) were subcloned into the pESC-URA yeast dual expression vector (Agilent, Santa Clara, CA, USA) according to the manufacturer’s protocol. Transformant selection and growth, as well as analyses of polyisoprenoid profile and CPY glycosylation status, were performed as described previously (Surowiecki, Onysk, Manko, Swiezewska, & Surmacz, 2019).