Homology Modelling of RpoE10:
The homology models of RpoE10 and its truncated versions RpoE10(Del1)
and RpoE10(Del2) were generated using the X-ray crystal structure
of Mtb-SigJ (PDB ID: 5XE7, chain B) as template 20. We
first constructed a model of the RpoE10 protein containing the SnoaL_2
domain and then modeled the deleted part of the loop. The model of
Mtb-SigJ was built with the Modeler module using the HHPRED server21. The quality and reliability of the built model
were evaluated using PROCHECK and Verify 3D programs. Structural
superposition and structure-based sequence alignment were performed
using the MatchMaker and MatchAlign tool of the UCSF Chimera package22. In the RpoE10 model, in silico substitution
of DGG to AAA in RpoE10(Mut1) and PDK to AAA in RpoE10(Mut2) was
generated using the rotamer tool of UCSF-Chimera tool22.