Figure 2.   Comparative plots of RMSD profile of (A) RpoE10 (B) RpoE10 (Mut1) and (C) RpoE10 (Mut2). RMSD plot was computed through the least square fitting of the backbone atom. Plots were calculated for 𝝈2(red), 𝝈4 (blue), SnoaL_2(yellow), 𝝈2-𝝈4 (violet), 𝝈2-𝝈4- SnoaL_2 (black), NPDKV (cyan), DGGGR (magenta), and WLPEP (green) motifs of linker strand (grey) segments for RpoE10, Rp0E10 (Mut1) and Rp0E10 (Mut2) models. Figure 2 D, E, and F (Right Panel): showing the Impact of SnoaL_2 domain mutations on the conformational dynamics .The RpoE10 (Mut1) and RpoE10 (Mut2) influence the backbone conformations of σ2 -σ4 domains. RpoE10 (Mut1) showed a more stable conformation of the σ2- σ4 domain than RpoE10 (Mut2) and RpoE10 across many conformers. Conformational heterogeneity can be seen in RpoE10 and RpoE10 (Mut2). The domain fluctuations and its impact in regulating the promoter activation is depicted in the schematic diagram.