Homology Modelling of RpoE10:
The homology models of RpoE10 and its truncated versions RpoE10(Del1) and RpoE10(Del2) were generated using the X-ray crystal structure of Mtb-SigJ (PDB ID: 5XE7, chain B) as template 20. We first constructed a model of the RpoE10 protein containing the SnoaL_2 domain and then modeled the deleted part of the loop. The model of Mtb-SigJ was built with the Modeler module using the HHPRED server21. The quality and reliability of the built model were evaluated using PROCHECK and Verify 3D programs. Structural superposition and structure-based sequence alignment were performed using the MatchMaker and MatchAlign tool of the UCSF Chimera package22. In the RpoE10 model, in silico substitution of DGG to AAA in RpoE10(Mut1) and PDK to AAA in RpoE10(Mut2) was generated using the rotamer tool of UCSF-Chimera tool22.