Construction of abm:gfp fusion:
The promoter region of rpoE10 up to ATG was amplified with the
help of forward (ProRpoE10GFP_FP) and reverse (ProRpoE10GFP_RP)
primers containing KpnI and NdeI site, respectively. The
purified insert was digested and ligated into a broad host range vector,
pBBR1MCS-3 (designated as pAPD10). The PCR amplified E-GFP was digested
with NdeI and XhoI restriction enzymes and ligated into
pAPD10, and the resulting construct was designated as pAPD11.