Figure 3. The radius of gyration (Rg) analysis.
Rg trajectory of A) RpoE10, B) RpoE10 (Mut1), and C) RpoE10 (Mut2) shown
as σ2 (red), σ4(blue), SnoaL_2(yellow), σ2-σ4 domain (purple), and
entire protein chain (black). The Average Rg value is written at the
right margin of the corresponding graph. RpoE10 (Mut1) showed a more
compact σ2- σ4 domain than RpoE10 (Mut2) and RpoE10. A plot of minimum
distance calculated between σ2 and σ4 domain for D) RpoE10, E) RpoE10
(Mut1), and F) RpoE10 (Mut2) from 200ns trajectory. Figure G, H,
and I depict the analysis of the compactness of the σ2-σ4 domain for G)
RpoE10, H) RpoE10 (Mut1), and I) RpoE10(Mut2. RpoE10 (Mut1) showed a
more compact σ2- σ4 domain than RpoE10 (Mut2) and RpoE10.