RpoE10 model Structure of A. brasilense:
The structural model of the RpoE10 of A. brasilense exhibited similar spatial arrangements to its template crystal structure of SigJ of M. tuberculosis (Mtb-SigJ; PDB ID: 5XE7) (Figure 1A). The overall architecture of RpoE10 can be structurally categorized into three discrete sub-domains, viz., the N-terminal σ2and σ4 domain, and the C-terminal SnoaL_2 domain. Global sequence alignment using the Needleman Wunsch tool of NCBI showed 36% sequence identity between RpoE10 and Mtb-SigJ. The target-template structures showed 44% sequence identity in the σ24domain sequences, excluding the C-terminal extension. The C-terminal extension of Mtb-SigJ and RpoE10 revealed 27% sequence identity. The σ2 and σ4 and SnoaL 2 are threaded to each other through a polypeptide linker strand connecting σ2 with σ4. The two conserved motifs200DGGGR204 and277NPDKV281 of Snoal_2 domain were in the vicinity (5Å zone) of the inter-domain linker strand, containing83WLPEP87 motif (Figure 1A; inset view).