Figure 2. Comparative plots of RMSD profile of (A)
RpoE10 (B) RpoE10 (Mut1) and (C) RpoE10 (Mut2). RMSD plot was computed
through the least square fitting of the backbone atom. Plots were
calculated for 𝝈2(red), 𝝈4 (blue), SnoaL_2(yellow), 𝝈2-𝝈4 (violet),
𝝈2-𝝈4- SnoaL_2 (black), NPDKV (cyan), DGGGR (magenta), and WLPEP
(green) motifs of linker strand (grey) segments for RpoE10, Rp0E10
(Mut1) and Rp0E10 (Mut2) models. Figure 2 D, E, and F (Right
Panel): showing the Impact of SnoaL_2 domain mutations on the
conformational dynamics .The RpoE10 (Mut1) and RpoE10 (Mut2) influence
the backbone conformations of σ2 -σ4 domains. RpoE10 (Mut1) showed a
more stable conformation of the σ2- σ4 domain than RpoE10 (Mut2) and
RpoE10 across many conformers. Conformational heterogeneity can be seen
in RpoE10 and RpoE10 (Mut2). The domain fluctuations and its impact in
regulating the promoter activation is depicted in the schematic diagram.