Bacterial strains, plasmid, chemicals, and growth conditions :
The E. coli DH5αand E. coli S17.1 were grown in Luria
Bertani (LB) medium at 37oC. A.brasilense Sp245
was grown in minimal malate (MM) medium or LB medium at
30oC. All the chemicals used for culturing bacteria
were from Hi-media (Mumbai, India), and enzymes used for DNA
manipulation and cloning were from New England Biolabs. All strains and
plasmids used in this work are listed in Table1.