RpoE10 model Structure of A. brasilense:
The structural model of the RpoE10 of A. brasilense exhibited
similar spatial arrangements to its template crystal structure of SigJ
of M. tuberculosis (Mtb-SigJ; PDB ID: 5XE7) (Figure 1A). The
overall architecture of RpoE10 can be structurally categorized into
three discrete sub-domains, viz., the N-terminal σ2and σ4 domain, and the C-terminal SnoaL_2 domain.
Global sequence alignment using the Needleman Wunsch tool of NCBI showed
36% sequence identity between RpoE10 and Mtb-SigJ. The target-template
structures showed 44% sequence identity in the
σ2-σ4domain sequences, excluding the
C-terminal extension. The C-terminal extension of Mtb-SigJ and RpoE10
revealed 27% sequence identity. The σ2 and
σ4 and SnoaL 2 are threaded to each other through a
polypeptide linker strand connecting σ2 with
σ4. The two conserved motifs200DGGGR204 and277NPDKV281 of Snoal_2 domain were
in the vicinity (5Å zone) of the inter-domain linker strand, containing83WLPEP87 motif (Figure 1A; inset
view).