Bacterial strains, plasmid, chemicals, and growth conditions :
The E. coli DH5αand E. coli S17.1 were grown in Luria Bertani (LB) medium at 37oC. A.brasilense Sp245 was grown in minimal malate (MM) medium or LB medium at 30oC. All the chemicals used for culturing bacteria were from Hi-media (Mumbai, India), and enzymes used for DNA manipulation and cloning were from New England Biolabs. All strains and plasmids used in this work are listed in Table1.