2.3 Histopathology:
Skin biopsy specimens of size 5-10mm were cut and fixed in 10% buffered formalin for 48 hours. The tissues were sequentially dehydrated in ethanol (65-100%) and cleared in three changes in xylene. The paraffin blocks were prepared and 5µm sections were prepared for staining with hematoxylin and eosin staining method. The histological lesions were documented under 10x and 40x objective in compound microscope (DM3000, Leica, Germany)