2.9 Identification of genes potentially involved in gall formation and host manipulation
One hundred forty-one proteins were identified from the saliva ofS. chinensis in a previous study (Yang et al., 2018). These identified proteins were used to identify genes potentially involved in gall formation and host manipulation. tBLASTN was used to search the corresponding genes in the S. chinensis genome with the 141 salivary proteins as queries (E-value ≤1×10−5, identify ≥ 50). The expression levels of salivary protein-encoding genes were quantified in three stages based on the RNA-seq data. Up-regulated genes in fundatrix were subject to GO and KEGG enrichment analyses using Omicshare CloudTools with default parameters (http://www.omicshare.com/).