Immunohistochemistry (IHC)
A granular immunoreactivity to anti‐Mycobacterium antibody was detected
within granulomas in case 9 (Figure 2).
In the spleen of cases 1, 2, 5, 6, and 11, variable sized round
“Hamazaki-Wesenberg-like” bodies were immunolabeled with PAB antibody
within macrophages forming the granulomas (Figure 3). The
Hamazaki-Wesenberg bodies are classically detected in human lymph nodes
from patients affected by granulomatous conditions. These spheroidal
bodies are interpreted as forms of intracellular bacteria that can be
detected by immunohistochemistry (Negi et al., 2012).