Histology and Histochemistry
The remaining parts of each organ sampled for molecular investigations
were fixed in 10% (vol/vol) phosphate‐buffered formalin according to
standard procedures, paraffin embedded, and then cut into 3μm sections.
Then, the sections were stained with Hematoxylin-Eosin (H&E).
Concurrently sections of the spleen were stained with Gram (Gram Kit,
Histoline Laboratories, Milan, Italy) and for the detection of acid‐fast
bacteria (Zeehl‐Neelsen Kit, Histoline Laboratories, Milan, Italy).
Archived positive controls for Gram and acid-fast staining were
included.