Immunohistochemistry (IHC)
A granular immunoreactivity to anti‐Mycobacterium antibody was detected within granulomas in case 9 (Figure 2).
In the spleen of cases 1, 2, 5, 6, and 11, variable sized round “Hamazaki-Wesenberg-like” bodies were immunolabeled with PAB antibody within macrophages forming the granulomas (Figure 3). The Hamazaki-Wesenberg bodies are classically detected in human lymph nodes from patients affected by granulomatous conditions. These spheroidal bodies are interpreted as forms of intracellular bacteria that can be detected by immunohistochemistry (Negi et al., 2012).