2.7 Statistical analysis
All data are expressed as the mean of four replicates ± standard error
(SE). Significant difference in the content or relative13C-enrichment throughout the time was determined by
one-way analysis of variance (ANOVA) and Dunnet test (P< 0.05), using the time 0 min as control. The difference
between dark and light treatments in each time point was determined by
Student’s t test (P < 0.05). These statistical
analyses were carried out using SIGMAPLOT12 (Systat Software Inc., San
Jose, CA, USA) or Minitab 18 statistical software (State College, PA:
Minitab, Inc.). Principal component analysis (PCA) was carried out in
square root transformed and auto-scaled (mean-centred and divided by the
standard deviation of each variable) data using the Metaboanalyst
platform (Chong et al. 2018).