2.4 Physiological analyses of cold-treated cassava plants
Malondialdehyde (MDA) content in cassava leaves was determined by using
Lipid oxidation detection kit (Beyotime) according to the manufacturer’s
instructions. MDA was extracted with chilled thiobarbituric acid
reagent, and then the MDA contents were calculated by subtracting the
absorbance at 450 and 600 nm from that at 532 nm. Proline concentrations
were measured by using Proline Assay Kit (Bioss, Beijing) according to
the manufacturer’s instructions. Total protein extraction, purification,
and determination were performed by Sanshu Biotechnology Co., Ltd
(Jiangsu, China). Each treatment had three replications.