Determination of Triacylglycerol Composition
The Triacylglycerol (TAG) composition of the fractionated pequi oil,
fractionated Kpangnan butter, and their binary mixtures were determined
using a Waters Alliance model 2690 high-performance liquid
chromatography (HPLC) with a refractive index detector (Waters model
2410, Waters, Milford, MA, USA). Chromatographic separation of the TAGs
in the samples was obtained with a Waters Xbridge C18 (Waters Limited,
Mississauga, ON, Canada) column (4.6 mm × 250 mm internal diameter with
5 μm particle size). Isocratic elution with a flow rate of 1 mLmin−1 of
degassed acetone/acetonitrile 60/40 (v/v) was applied. Column and
detector temperatures were set at 40 oC. The separated
peaks were analyzed using Millenium 32 software (K&K Testing, LLC,
Decatur, GA, USA). The TAG composition was determined using internal
standards and their corresponding retention times.