2.10 RNA isolation and real-time PCR
Total RNA was extracted from fresh renal tissue by using the TRIzol
method and then reverse transcribed into cDNA. The cDNA content was
detected by real-time quantitative PCR to determine the gene
transcription levels in the tissue. Samples from five independent
experiments were utilized, and each sample was subjected to real-time
PCR in triplicate. The primers used in this study are provided in Table
S1.