2.1 Materials
Leonurine (98.24%) was purchased from PuFeiDe Biotech Co. (Chengdu,
China). Erastin and RSL3 were purchased from Selleckchem (Shanghai,
China). Cisplatin was purchased from Med Chem Express (New Jersey, USA).
Primary antibodies against β-actin, KIM1, NGAL, GPX4, XCT, FTL, FTH-1,
TFR, Nrf2, HO-1, and NQO1 were purchased from Abcam (Cambridge MA, USA),
Cell Signaling Technology (Danvers, Massachusetts, USA), R&D Systems
(Minnesota, USA) and Proteintech (Wuhan, China). The BCA protein assay
kit was purchased from Thermo Fisher Scientific (MA, USA). The iron
assay kit was purchased from BioAssay Systems (Northern California Bay
Area, USA). Calcein-acetoxymethyl (AM) was purchased from Beyotime
Biotechnology (Jiangsu, China). DMEM/F-12, penicillin, streptomycin and
trypsin EDTA, used for cell culturing, were purchased from hyclone
(Logan, Utah, USA). Dimethyl sulfoxide (DMSO) and DCFH-DA were purchased
from Sigma (St. Louis, Missouri, USA). Cell Counting Kit-8 (CCK-8) was
purchased from Bimake (Houston, USA). Nrf2 siRNA was obtained from
Hanbio Biotechnology Co., Ltd. (Shanghai, China). SYBR Green I was
purchased from Roche (Basel, Switzerland).