2.1 Materials
Leonurine (98.24%) was purchased from PuFeiDe Biotech Co. (Chengdu, China). Erastin and RSL3 were purchased from Selleckchem (Shanghai, China). Cisplatin was purchased from Med Chem Express (New Jersey, USA). Primary antibodies against β-actin, KIM1, NGAL, GPX4, XCT, FTL, FTH-1, TFR, Nrf2, HO-1, and NQO1 were purchased from Abcam (Cambridge MA, USA), Cell Signaling Technology (Danvers, Massachusetts, USA), R&D Systems (Minnesota, USA) and Proteintech (Wuhan, China). The BCA protein assay kit was purchased from Thermo Fisher Scientific (MA, USA). The iron assay kit was purchased from BioAssay Systems (Northern California Bay Area, USA). Calcein-acetoxymethyl (AM) was purchased from Beyotime Biotechnology (Jiangsu, China). DMEM/F-12, penicillin, streptomycin and trypsin EDTA, used for cell culturing, were purchased from hyclone (Logan, Utah, USA). Dimethyl sulfoxide (DMSO) and DCFH-DA were purchased from Sigma (St. Louis, Missouri, USA). Cell Counting Kit-8 (CCK-8) was purchased from Bimake (Houston, USA). Nrf2 siRNA was obtained from Hanbio Biotechnology Co., Ltd. (Shanghai, China). SYBR Green I was purchased from Roche (Basel, Switzerland).