Replication competent (RC) AAV2 infection
Initial stocks of RC AAV2 were generated via plasmid transfection and titrated by dPCR. When using RC AAV2 infection as a positive control for capsid assembly, HEK-293SF cells at an initial cell density of 106 cell/ml were infected with RC AAV2 stocks and hAd5 and an MOI of 10.