Figure 1. Production of rAAV2-GFP by plasmid transfection
HEK-293SF cells in serum-free suspension were transfected with equimolar amounts ofpAdDeltaF6, pAAV-RC2, and pAAV-CMV-GFP to produce AAV2 vectors carrying a GFP transgene. a) Cell density in transfected and mock transfected cells tracked until harvest at 48 hpt. b) Volumetric vector yield in terms of nuclease-resistant vector genomes measured by digital droplet PCR. Error bars represent SEM for n=4 biological replicates.