HEK-293SF cells producing AAV2-GFP vectors by transfection were fixed 48 hpt and stained for assembled AAV capsids. a) Gating for transfection marker (GFP) and assembled AAV capsids following exclusion of debris and singlet gating (not shown). Also shown are mock transfected negative controls and a positive control for capsid assembly consisting of cells infected with replication competent (RC) AAV2 with a hAd5 helper virus b) Quantification of flow cytometry results from n=3 biological replicates. Error bars represent SEM.