Figure 1. Production of rAAV2-GFP by plasmid
transfection
HEK-293SF cells in serum-free suspension were transfected with
equimolar amounts ofpAdDeltaF6, pAAV-RC2, and
pAAV-CMV-GFP to produce AAV2 vectors carrying a GFP transgene. a) Cell
density in transfected and mock transfected cells tracked until harvest
at 48 hpt. b) Volumetric vector yield in terms of nuclease-resistant
vector genomes measured by digital droplet PCR. Error bars represent SEM
for n=4 biological replicates.