Flow cytometry
Cells were fixed and permeabilized as previously described (Kerviel et al., 2016). Flow cytometry was carried out on the BD FACSJazz (BD Biosciences) and analyzed on FlowJo v10. Assembled particles of AAV2 were detected by staining with anti A20R (Progen) labelled with AlexaFluor 594 (Invitrogen).