AAV genome quantification by digital droplet PCR
HEK-293SF cells in culture media were freeze-thawed three times to lyse
cells. Clarified lysates were then incubated with 5U/ml Benzonase to
digest unencapsulated DNA. Encapsulate genomes were then purified with
the High Pure Viral DNA Extraction kit (Roche Diagnostics) and
quantified on the QX200 Digital Droplet PCR system (Bio-Rad) as
previously described (Furuta-Hanawa, Yamaguchi, & Uchida, 2019).