Replication competent (RC) AAV2 infection
Initial stocks of RC AAV2 were generated via plasmid transfection and
titrated by dPCR. When using RC AAV2 infection as a positive control for
capsid assembly, HEK-293SF cells at an initial cell density of
106 cell/ml were infected with RC AAV2 stocks and hAd5
and an MOI of 10.