Plasmids
For the generation of rAAV2, plasmids pAdDeltaF6 (Addgene #112867),
pAAV-RC2 (cell biolabs inc.), and pAAV-CMV-GFP (Addgene #67634) were
transfected in a 1:1:1 molar ratio. pAdDeltaF6 was a gift from James M.
Wilson and pAAV-CMV-GFP was a gift from Connie Cepko (Xiong, MacColl
Garfinkel, Li, Benowitz, & Cepko, 2015). Stocks of RC AAV2 were
generated by equimolar transfection of pAdDeltaF6 and pAV2-Cla.
pAV2-Cla, which contains a sequence identical to wildtype AAV2 apart
from a point mutation to generate a Cla1 restriction site in the 3’ UTR.
pAV2-Cla was generously provided by Dr. Thomas Webber.