Statistical analysis
The data and statistical analysis
comply with recommendations for experimental design and analysis in
pharmacology (Curtis et al., 2015, 2018). Statistical analysis was
performed with Prism 7 software (GraphPad Prism, La Jolla, USA). All
data are expressed as the mean ± standard error of the mean (SEM). We
used a two-way (time and pharmacological treatment or time and genotype)
analysis of variance (ANOVA) followed by the Bonferroni post-hoc test
for multiple comparisons. For other analyses, we used a Mann-Whitney
test. Data were considered statistically significant when p was
< 0.05.