Figure 6 Cav3.2 channels are involved in the
activation/proliferation of macrophages and T CD4+lymphocytes
Immunohistochemistry of BMDM (actin [phalloidin toxin]= red) and
nucleus (DAPI= blue)) from WT and Cav3.2 KO mice
(A ). BMDM area (B, left ) and IL-6 production in
supernatant (B, right ). (C ) Representative recording
of LPS-induced calcium signals in single WT and Cav3.2
KO BMDM (left ) and percentage of BMDM responding to LPS
(right ) (515 cells analyzed for WT and 446 for
Cav3.2 KO BMDM from 4 different mice/group).
Flow cytometry analysis of the activation status (CD86 median
fluorescence intensity) in macrophages (D, left ), dendritic
cells (DC CD11b+, D, middle ) and inflammatory
monocytes (D, right ) from spleen of WT and
Cav3.2 KO mice. (E ) Proliferation capacity of
WT and Cav3.2 KO T cells stained with CFSE under
CD3/CD28 stimulation for 4 days. Representative flow cytometry data
showing CFSE peaks displayed by CD4+ T cells
(E, left ). Quantification of T CD4+ cells
after proliferation (E, middle and right ). B left and
right and E middle were analyzed by one-way ANOVA followed by
Bonferroni post-hoc test (*p < 0.05; ***p< 0.001). Other graphs were analyzed by Mann-Whitney test
(**p < 0.01; ***p < 0.001). Data
represent mean ± SEM.