Figure 6 Cav3.2 channels are involved in the activation/proliferation of macrophages and T CD4+lymphocytes
Immunohistochemistry of BMDM (actin [phalloidin toxin]= red) and nucleus (DAPI= blue)) from WT and Cav3.2 KO mice (A ). BMDM area (B, left ) and IL-6 production in supernatant (B, right ). (C ) Representative recording of LPS-induced calcium signals in single WT and Cav3.2 KO BMDM (left ) and percentage of BMDM responding to LPS (right ) (515 cells analyzed for WT and 446 for Cav3.2 KO BMDM from 4 different mice/group).
Flow cytometry analysis of the activation status (CD86 median fluorescence intensity) in macrophages (D, left ), dendritic cells (DC CD11b+, D, middle ) and inflammatory monocytes (D, right ) from spleen of WT and Cav3.2 KO mice. (E ) Proliferation capacity of WT and Cav3.2 KO T cells stained with CFSE under CD3/CD28 stimulation for 4 days. Representative flow cytometry data showing CFSE peaks displayed by CD4+ T cells (E, left ). Quantification of T CD4+ cells after proliferation (E, middle and right ). B left and right and E middle were analyzed by one-way ANOVA followed by Bonferroni post-hoc test (*p < 0.05; ***p< 0.001). Other graphs were analyzed by Mann-Whitney test (**p < 0.01; ***p < 0.001). Data represent mean ± SEM.