Bone-marrow derived macrophages and lymphocytes T CD4+ cells
Bone-marrow derived macrophages (BMDM) and CD4+ T cells were prepared from WT and Cav3.2GFP-flox KI mice and used to investigate the expression of Cav3.2 channels by immunohistochemistry labelling. BMDM from WT and Cav3.2 KO mice was used to investigate the impact of Cav3.2 deletion on LPS-induced morphological modification, IL-6 secretion and intracellular calcium by immunofluorescent labelling, ELISA, and calcium imaging. CD4+ T cells from WT and Cav3.2 KO mice were used to assess the proliferation capacities after CD3/CD28 activation by flow cytometry.