Figure 5 Anti-inflammatory effect of Cav3.2
channels inhibition is mediated by immune cells
Edema development after carrageenan injection in
Cav3.2Nav1.8 cKO and
Cav3.2GFP-flox KI mice (A ).
(B ) Representation of chimeric mice generation. WT and
Cav3.2 KO mice (recipients) are irradiated. In
irradiated mice, bone marrow cells retrieved from un-irradiated WT or
Cav3.2 KO mice (donors) were transplanted by intravenous
injection. Four experimental groups were obtained: WT mice transplanted
with bone marrow cells from Cav3.2 KO;
Cav3.2 KO mice transferred with bone marrow cells from
WT donors; WT mice transferred with bone marrow cells from WT donors;
and Cav3.2 KO mice transferred with bone marrow cells
from KO donors. Edema development (C ) and IL-6 production
(D ) in chimeric mice 4 hours after injection of carrageenan, 8
weeks after the bone marrow transplantation. (E ) BMDM and
CD4+ T cells, obtained from WT and
Cav3.2GFP-flox KI mice stained with
antibody anti-eGFP or isotype control revealing the expression of
Cav3.2 in these cells.B was analyzed by one-way
ANOVA followed by Bonferroni post-hoc test: no significant differences
were found. For other graphs a one-way ANOVA followed by Bonferroni
post-hoc test were performed (*p < 0.05; **p< 0.01; ***p < 0.001). Data represent mean ±
SEM.